November 5‚ 2014 Bioinformatics Leigh Ann Santana DNA Extraction Lab DNA extraction is an important process because the DNA first needs to be purified away from proteins and other cellular contaminants. Cell are needed‚ because that is where the DNA is located. Inside almost every cell in our bodies is a nucleus‚ and inside each nucleus is about two meters of DNA. The following steps are needed to purify DNA from a cheek swab. Collect cheek cells‚ Burst cells open to release DNA‚ separate DNA
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DETERMINATION OF COPPER BY COMPLEXATION‚ SOLVENT-EXTRACTION AND SPECTROPHOTOMETRY ABSTRACT To determine the concentration of copper in an unknown solution by using copper complexation‚ solvent extraction and spectrophotometry. Standards are used to create a calibration curve and the unknown concentration of copper is then calculated by using the linear equation from the calibration curve. The concentration of copper in the unknown solution 201 was found to be 12.57± 0.25 μg/mL. INTRODUCTION Copper
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BINARY COMPOUNDS -Compose of two kinds of atom Polyatomic Ion | Name | OH-1 | hydroxide | SO4-2 | sulfate | PO4-3 | phosphate | NO3-1 | nitrate | CO3-2 | carbonate | HCO3-1 | hydrogen carbonate or bicarbonate | ClO3-1 | chlorate | NH4+1 | ammonium | TWO NON-METALS - Give the name of the positive ion followed by the negative ion ending with -ide -Greek prefixes are used CO-carbon monoxide CO2-carbon dioxide NO-nitrogen
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Supercritical Carbon Dioxide as a Green Solvent Gregory Absillis June 2009 The majority of chemical processes in chemical synthesis are carried out in solution and organic solvents usually are the first choice. To satisfy this demand‚ 15 billion kilograms of organic are produced worldwide each year. [1] These solvents often bear considerable risk due to their toxicity‚ flammability or environmental hazards. Furthermore‚ they have to be separated from the products and recycled or disposed at
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based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or liquid (solvent; eluent) • For liquids
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Name: Leung Chun Wai Student no.: G20554292 Topic: (NT3048) Smoke extraction design for large space such as atrium 1. INTRODUCTION Atrium inside a building is an old architectural concept for about two thousand years. This concept has been developed and extended to modern buildings. Current trend of atrium design provides an ‘ideal’ external environment to the occupants with a large undivided space. The major problem for fires in atrium is hot and toxic gases accumulating and go down in the atrium
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Extraction of caffeine from Lipton tea leaves Maria Gianna Beatrice L. Cancio*‚ Joe Mari Isabella B. Caringal‚ Rowena A. Chiang‚ Patricia Deanne del Valle Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España‚ Manila‚ Philippines Abstract Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three
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Santo Tomas Faculty of Pharmacy Organic Chemistry Laboratory RECRYSTALLIZATION OF ACETANILIDE USING WATER AS SOLVENT Lagarteja‚ M.C.B.; Lim‚ H.G.N.; Lizo‚ K.J.R.; *Macalino‚ M.D.L.; Macapala‚ C. 2D-Pharmacy‚ Faculty of Pharmacy‚ University of Santo Tomas Abstract Recrystallization is a technique used to purify organic solids. This method involves dissolving of a solute in a solvent and inciting the solute to produce a precipitate from a solution. In this experiment‚ acetic anhydride was added
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Organic Chemistry/Biochemistry 1: Compounds Important to Life • Biologists classify compounds into organic compounds and inorganic compounds. Organic Compound: a compound that is derived from living things and contains carbon Inorganic Compound: a compound generally derived nonliving things • Water Water is an inorganic molecule with unique properties that make it one of the most important compounds for living things. In the water molecule (H2O)‚ the hydrogen and oxygen atoms bond
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Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh‚ Soft Fruit (e.g.‚ Grapes‚ Strawberries‚ Banana‚ etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou Must ProvideContains sodium chloride‚ detergent and waterFor ice cold ethanol‚ store in the freezer 60 minutes before use. Procedure If you have not done so‚ prepare the ethanol by placing it in a freezer for approximately
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