DNA extraction lab 1. A number of steps are required to isolate DNA from cellular content. Describe what happens at each step‚ and why it acts to separate the parts of the cell. The steps include a) breaking cell open to release the DNA; b) separating the DNA from cellar materials and proteins; c) using alcohol to precipitate the DNA; d) cleaning the DNA; e) confirming the presence of the DNA. a) Breaking cell open to release the DNA: the cells are separated from each other by physical means such
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Compare and Contrast ‘Neutral Tones’ by Thomas Hardy and ‘Absence’ by Elizabeth Jennings. In both poems Thomas Hardy and Elizabeth Jennings they have lost a person they were close to. There are some similarities in their feeling but there responses are different. Neither poets can forget the experience and are reminiscing on the situation. They are both going back to the place where they were with the people they loved. However in ‘Absence’ she has actually gone to the place they were together
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Extraction of R’-NH2 For both of the organic acid and organic base extraction‚ the addition of the respective inorganic acid or inorganic base (HCl or NaOH) to the three-component mixture caused an immiscible appearance of the solution in the separatory funnel. By briskly shaking the separatory funnel with the mixed components created a build up of pressure which was released through the stopcock forming small disappearing bubbles within the tip of the separatory funnel. Once the funnel was placed
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COORDINATION COMPOUNDS: * FLEXIDENTATE CHARACTER OF POLYDENTATE LIGANDS; * Poly dentate ligands have flexi dentate character in the sense that all donors atoms may not form coordinate bonds with the central metal atom or ion. For example; EDTA which is the hexa dentate ligand also acts as tetra dentate or penta dentate in certain complexes. Similarly sulphate ion‚ which is a bi dentate ligand‚ also acts as a mono dentate ligand in certain complexes‚ e.g. in [Co(SO4)(NH3)5]Cl. * LABILE
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(Sucrose)- C12H22O11 Vitamin C (Ascorbic Acid)- C6H8O6 Aspirin (Acetylsalicylic Acid)- C9H8O4 Common Name | Chemical Name | acetone | dimethyl ketone; 2-propanone (usually known as acetone) | acid potassium sulfate | potassium bisulfate - used in the conversion of tartrates to bitartrates in wine. | acid of sugar | oxalic acid – used in cleaning or bleaching‚ especially for the removal of rust (iron complexing agent) | ackey | nitric acid | alcali volatil | ammonium hydroxide
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Gender Neutral Bathrooms Subjecting others to labels‚ whether accurate or not‚ is a criminal practice that happens daily. The practice of putting something as complex as a human being into a neat‚ little box is a gross understatement of human worth. In the article “No more Women’s Rooms: Why bathrooms should be gender neutral”‚ by Izzy Rode‚ it was established that most transgender people do not feel accepted in whichever bathroom they go into due to a perceived misconception on what it means to
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What is a neutral curriculum and can teachers truly leave their beliefs at the door of their classrooms upon entering? To tell the truth‚ this is a question I have pondered on myself for years. My answer to this question is and always has been‚ NO! A neutral curriculum‚ in my opinion‚ is one that lacks any thought on morals‚ character education‚ or a teacher’s religious beliefs. I have yet to meet a teacher who does not‚ on a daily basis‚ interject some kind of personal beliefs about classroom
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The purpose of the DNA extraction lab was not only to inform students on how DNA is present in humans and all organisms‚ but to also educate them on how DNA can be extracted using common household materials. Also‚ the lab was very efficient as it introduced the students to extracting their own DNA found on their cheek cells as well as letting them take an observation on how DNA appears or how it is formed. Additionally‚ students were instructed through a very clear and simple procedure‚ which guided
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Liquid CO2 Extraction of D-Limonene This convenient‚ effective and inexpensive liquid CO2-based extraction of a natural product (D-limonene) provides an engaging example of natural products extraction using an alternative solvent (liquefied CO2). Because the procedure can be carried out in a polypropylene centrifuge tube and uses crushed dry ice as its CO2 source‚ it provides an inexpensive alternative to the equipment-intensive extraction with supercritical CO2. A short video clip is available
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Lab 6: Organic Compound Analysis Isabella O’Toole CHY 116 Friday 1:15-4:00 Lab Conducted on 4-12-13 and 4-19-13 4-26-13 This lab was conducted in order to determine the chemical composition of an unknown organic acid. A known mass of acid was dissolved into 30mL of either water or methanol (depending on solubility) and titrated with standardized sodium hydroxide. Data from this allowed the molar
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