TWO-BASE EXTRACTION OF BENZOIC ACID‚ 2-NAPHTHOL‚ AND NAPHTHALENE FROM UNKNOWN SAMPLE # 131 Douglas G. Balmer (T.A. Mike Hall) Dr. Dailey Submitted 11 July 2007 Introduction: The purpose of this experiment was to separate a sample of benzoic acid‚ 2- naphthol‚ and naphthalene of unknown proportions using a two-base extraction method. The three components of the mixture will react differently to sodium bicarbonate and sodium hydroxide because each of the bases’ conjugate acids has a different
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Extraction of DNA Science Fair Project 2013-2014 Produced by Nadia Walker Due Date: Monday January 20th 2014 Presented to Mr. Freimann Date Submitted: Monday January 20th 2014 Table of Contents Introduction page 1 Hypothesis and materials
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Assignments in Science Class X (Term II) 4 Carbon and its Compounds IMPORTANT NOTES 9. Unsaturated organic compounds : Organic compounds in which a double or a triple bond exists between two carbon atoms in a carbon chain‚ are called unsaturated organic compounds. 10. Hydrocarbons : Organic compounds which contain only carbon and hydrogen atoms are called hydrocarbons. 11. Straight chain hydrocarbons : Hydrocarbons‚ in which all the carbon atoms are linked to one another in a straight chain
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the quantification of albumin and casein were performed and analyzed after extraction of the said proteins from their respective sources. Isolation of proteins was initiated by the breakage of the cell wall / membranes in three different ways. Homogenization of invertase‚ albumin and casein were achieved via grinding process‚ addition of 1M acetic acid and acidification by 0.1M hydrochloric acid correspondingly. Extraction of invertase and casein involved precipitation through the utilization of 95%
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stereochemistry General Concepts The most common of the variety of methods available for preparing alkyl halides is the replacement of the hydroxyl group of an alcohol. This type of reaction is representative of an important class of reactions in organic chemistry called nucleophilic aliphatic substitution reactions. There are numerous ways of doing this transformation‚ and we will discuss these in lecture. In this experiment you will convert an alcohol to an alkyl halide by reaction with a hydrohalic
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Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
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Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
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Music Appreciation April 30‚ 2013 “The Separation” The separation of church music and secular music during the Baroque period was the beginning of something new. Many people had no idea that the instruments they used during the Renaissance period could do more than they had been doing with them. This brought a new meaning of music and life into the world. It was a great change for music‚ instruments‚ and even people. Church music‚ also known as sacred music‚ was well known in the Renaissance
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Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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1. An organic compound X with a molecular formula C2H6O undergoes oxidation with in presence of alkaline KMnO4 to form a compound Y. X on heating in presence of Conc. H2SO4 at 443K gives Z. Which on reaction with H2Oin presence of H2SO4 gives back `X` `Z` reacts with Br2 (aq) and decolorizes it. Identify X‚ Y‚ & Z and write the reactions involved. 2. An organic compound ‘A’ is widely used as a preservative in pickles and has a molecular formula C2H2O2. This compound reacts with ethanol to
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