Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Separation by difference in particle size: Sieving: - Is a way pf separating mixtures of solids‚ or solids and liquids with different sized particles - The mixture is poured through a sieve and the particles smaller than the sieve pass through while the larger particles are trapped by the sieve - The are commonly used for cooking - E.g. a mixture of rice and water is poured through a sieve to capture rice and remove the water Filtration - Is a technique commonly used for mixtures of solids and liquid
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Separation Lab Purpose: This lab was based on the separation of the components of a mixture. A mixture can be defined as a physical combination of two or more pure substances. Separation techniques are used to separate components that are not chemically combined. All of these techniques involve changes in the physical state of a chemical compound rather than chemical changes. Sea sand‚ table salt‚ and two other unknown substances will be separated using the separation techniques in order to demonstrate
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Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
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I. INTRODUCTION For a stable political system‚ the power holders must be balanced off against each other. Doctrine of Separation of Powers‚ divides the political authority of the state into three distinct branches‚ namely: 1.) The Executive‚ 2.) The Legislature‚ and 3.) The Judiciary. The following three organs of the government represent the people and their will in the country and are responsible for the smooth running of the government. This principle deals with the mutual relations among these
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Abstract By separating chemicals out of a mixture and purifying them through sublimation and recrystiliation‚ changes in the substances physical properties can be shown. A salicylic acid-naphthalene mixture was mixed together with diethyl ether and saturated sodium bicarbonate. Two layers separated and were extracted after acidifying with HCl. This precipitated the crude salicylic acid. The left over mixture was combined with water and the two layers separated where crude naphthalene was recovered
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Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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Separation of a Mixture of Solids Lab: Separation of a Mixture of Solids Experiment PURPOSE The purpose of this experiment was to learn how to separate a mixture of solids. DATA | | |Data Table 1: Experiment Data | | |Grams |Percent of mixture | |Iron filings |2.2
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at least 29% of Canadian citizens have reported that they have faced family separation and divorce from their parents. Moreover‚ the statistics also showed that the average marriage in Canada only lasted for ten years at most (Urquia et al‚ 2013). A further fifteen percent admitted that separated spouses who shared time among both of them to raise them independently were raising them. The statistics of family separation in Canada are also quite serious because 50% of the people who are married believe
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