possible to identify which peak correlates with which compound. Figure 2 contains a peak at around 500 and 700 nm which is a good indicator that that would-be chlorophyll b. This makes sense because this was the bottom band from the thin layer chromatography and chlorophyll b is the most polar compound. Chlorophyll B contains an aldehyde where chlorophyll A has a methyl group making it slightly more polar. Following that‚ figure 4 contains
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Experiment 2: Dehydration of an Alcohol: Distillation and Gas Chromatography Preparation of Methylcyclohexenes Purpose: The basic purpose of this experiment is to carry out the dehydration of an alcohol and isolate the reaction products by distillation. Gas Chromatography will be utilized to analyze the reaction mixture. Table of Reagents: Compound (g) Molecular Weight (g/mol) Grams (g) Moles 6 mL of 2-methylcyclohexanol (C7H14O) 114.19 g/mol 6 mL x 0.943g = 5.66 g 1 mL 5.66 g x
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Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
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Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
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Task 3: How could Thin Layer Chromatography (TLC) have been used to prepare a pure sample of aspirin? The method of using thin layer chromatography is easy‚ quick and relatively cheap to carry out this makes it ideal for preparing pure samples of aspirin. Equipment Required: • Thin Layer Chromatography Paper • Ruler • Pencil • Solvent • Beaker • Micro Capillary • Access to a fume cupboard • UV light • Clingfilm • Individuals sample of aspirin • Pure sample of aspirin • Sample of salicylic acid Method:
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Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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Thin Layer Chromatography of Unknown Samples and Paprika By: Uyen Huynh Date: June 18‚ 2014 Lab Partners: Philip Murray Professor and section: M. Pandey‚ CHM2210L.601 Data: Analysis of Drug Store Items Knowns Rf Values Aspirin .91 cm Acetaminophen .85 cm Ascorbic Acid .86 cm Unknown .97 cm Identity of unknown mixture Acetaminophen Analysis of Paprika Pigments Spot Number Rf Color 1 1 Red-orange 2 1 Red-orange Calculations
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Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe
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in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front (mm) Rf Value Color A 6.0 57.0 0.22 Bluish green B 17.5 56.0 0.105 Dark green blue C 4.0 57.0 0.070 Greenish blue D 4.0 47.0 0.085 Green E 10.5 56.5 0.186 Light blue Figure 1: Chromatography- TLC sheet From left to right- acetic acid‚ citric acid‚
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For many divorced and separated parents with children there will be a common answer as to why they ended up with the child custody and visitation arrangement they have. The answer being‚ "the judge decided it." In other words‚ the judge chose the parenting arrangement you have based on his/her belief of what was in your child’s best interest. Judges do not always make the child custody decision or choose the parenting plan arrangement for the parent’s. In fact‚ more often than not‚ the judge will
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