Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Thin Layer Chromatography of Unknown Samples and Paprika By: Uyen Huynh Date: June 18‚ 2014 Lab Partners: Philip Murray Professor and section: M. Pandey‚ CHM2210L.601 Data: Analysis of Drug Store Items Knowns Rf Values Aspirin .91 cm Acetaminophen .85 cm Ascorbic Acid .86 cm Unknown .97 cm Identity of unknown mixture Acetaminophen Analysis of Paprika Pigments Spot Number Rf Color 1 1 Red-orange 2 1 Red-orange Calculations
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Plant cells are eukaryotic cells that differ in several key aspects from the cells of other eukaryotic organisms. Their distinctive features include: A large central vacuole‚ a water-filled volume enclosed by a membrane known as the tonoplast maintains the cell’s turgor‚ controls movement of molecules between the cytosol and sap‚ stores useful material and digests waste proteins and organelles. A cell wall composed of cellulose and hemicellulose‚ pectin and in many cases lignin‚ is secreted by
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Bacteria were among the first life forms to appear on Earth‚ and are present in most habitats on the planet‚ growing in soil‚ water‚ acidic hot springs‚ radioactive waste‚ and deep in the Earth’s crust‚ as well as in organic matter and the live bodies of plants and animals‚ providing outstanding examples of mutualism in the digestive tracts of humans‚ termites and cockroaches. Bacteria are microscopic living things that were among the first creatures to inhabit the earth. They can be found almost anywhere
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STATE OF CALIFORNIA DEPARTMENT OF TRANSPORTATION ENGINEERING SERVICES PRESTRESS MANUAL A GUIDE FOR FIELD INSPECTION OF CAST-IN-PLACE POST-TENSIONED STRUCTURES JANUARY 2005 Revision 1 05/05 TABLE OF CONTENTS Topic INTRODUCTION SAFETY PRESTRESS WORKING DRAWINGS PRESTRESSING DUCTS PRESTRESSING STRANDS/BARS ANCHORAGE DEVICES STRAND WEDGES PRESTRESSING JACKS PRESTRESSING OPERATION a. b. c. d. Preparation for Stressing Inspection Field Inspection Overstressing of Prestressing Steel Elongation
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knowledge‚ to my co-trainees who became part of my daily activities in my on-the-job training‚ and to my friends who were always there giving me comforting words and advices to work hard. Acknowledgement The completion of this manual could not have been possible without the participation and assistance of so
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that because substances have different properties‚ such as mass‚ that can be separated by chromatography. In our experiment‚ we chose 4 different color source to separate in water. We made sure that all the sources were water soluble because only polar substances will dissolve in water. We chose a black wet-erase marker‚ a red marker‚ a green marker‚ and mixed food coloring to test. The chromatography paper was split into 4 sections‚ about 2 cm above the bottom edge and evenly spaced out. Each section
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HENNES & MAURITZ INC. GLOBAL MARKET HEADQUATERS: SWEDEN About H & M According to the data published on the official website‚ H&M was established in Vasteras‚ Sweden in 1947 by Erling Persson. At the very beginning‚ when the first store was opened‚ it was actually given the name Hennes (the Swedish word for “hers”) because only women’s clothes were sold there at that time. Later in 1968‚ Erling Persson bought Mauritz Widforss‚ a hunting and gun store in Stockholm. Just from then
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information from http://www.researchandmarkets.com/reports/561970/ Management‚ 9th Edition Description: Management 9e‚ by John Schermerhorn‚ is based on constructive balances that are essential for success in business and management: the balance of managerial theory and practice; the balance of managerial ethics and positive action; and the balance of present realities with future possibilities. In this new edition‚Management‚ 9e has been extensively revised with a sincere commitment to help instructors
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possible to identify which peak correlates with which compound. Figure 2 contains a peak at around 500 and 700 nm which is a good indicator that that would-be chlorophyll b. This makes sense because this was the bottom band from the thin layer chromatography and chlorophyll b is the most polar compound. Chlorophyll B contains an aldehyde where chlorophyll A has a methyl group making it slightly more polar. Following that‚ figure 4 contains
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