Date Submitted: Sept. 2‚ 2014 EXPERIMENT NO. 1 EXTRACTION AND ISOLATION OF PROTEINS Background of the experiment Pure proteins are not readily available for study or use. There are certain methods that should be done in order to obtain proteins from its sources. The objective of this experiment is to isolate casein from milk and albumin from egg and to identify and explain the principles behind the methods employed for protein extraction. Results and Discussion There are many possible sources
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DNA Extraction of a Musa acuminata Biology Lab Report \Analyze and Interpret A number of steps are required to isolate DNA from cellular contents. Describe what happens at each step‚ and why it acts to separate the parts of the cell. /6 There are three specific steps required to isolate DNA from its cellular contents. The steps used to remove and expose DNA from its cell are: breaking down the food type you are using by crushing it‚ for example a banana or strawberries‚ exposing the substance
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Chromatographic Separation of Sodium Fluorescein and Methylene Blue In this two-part lab the students will: • Learn the techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for
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BASIC SEPARATION TECHNIQUES Contemporary modifications of filtration and dialysis use a matrix-based fibrous material that provides a mechanism of separation in many homogeneous immunoassays. These materials may be coated with specific antibody-ligand to foster selection of specific materials or species. Certain labels use magnetic particles in conjunction with strong magnets to effect separation. Basic universally used separation mechanisms‚ outside of those incorporated in immunoassay‚
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DNA Extraction Lab Problem Statement: Do you think you have ever eaten DNA? Background Information: DNA is too small to see under a regular microscope‚ so how can it be studied? DNA is a large molecule found in all living things; therefore it is possible to extract it from cells or tissues. All we need to do is disrupt the cell’s plasma membrane and nuclear envelope‚ make the DNA clump together and - voila! - DNA extraction is possible. DNA extractions from onion‚ bananas‚ liver‚ or wheat
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by everyday pollution from houses‚ factories‚ cars‚ and etc. This is the concept of acid rain‚ which according to EPA.gov is a broad term referring to a mixture of wet and dry deposition from the atmosphere containing higher than normal amounts of nitric and sulfuric acids. Acid rain isn’t just rain‚ its forms like hail or snow‚ which is wet deposition‚ and smoke or dust‚ which is dry deposition. The effects of acid rain are quite terrifying bringing all types of problems to the Earth‚ for example
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Separation of a Mixture of Solids I really enjoyed this lab. After reading the introduction I started by making my own flow sheet of how I thought this lab would work. I was reminded about solubility which is defined as the amount of the solute that will dissolve in a given amount of solvent and that the extent to which a substance dissolves depends mainly upon the physical properties of the solvent and of the solute and to some extent upon the solvent’s temperature. With that being said I came
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EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine
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Introduction DNA (Deoxyribose Nucleic Acid) is a nucleic acid that has many names‚ each representing the phases that it undergoes (chromosomes‚ chromatin‚ genes/alleles); it resides in the nucleus (bound by 2 *phospholipid bilayers) of almost every cell in the body (red blood cells being an exception). DNA (your genotype) is double stranded and is responsible for replicating (from 46 to 92) during Interphase‚ so that mitosis can make new cells‚ repairing and allowing for growth in the body. It is
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Abundance and Extraction of Metals 1. a Mineral - A mineral is a naturally occurring inorganic solid‚ with a definite chemical composition‚ and an ordered atomic arrangement. b Ore - Deposit in the Earth of valuable metals contained in minerals. c Economic Natural Resource Deposit - This is a natural deposit of something that is economically viable to mine‚ refine and sell. Hence the deposit must be big enough to last for a large period of time‚ so that it will make enough money for the
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