Extraction of DNA from Cheek Cells Gene Smith February 29th 2013 INTRODUCTION DNA‚ deoxyribonucleic acid‚ is the genetic material of every living organism and is found in the nucleus of eukaryotic cells. DNA is often called the ‘blueprint for life’ because it contains the necessary information to carry out all the living processes of the cell (1).The purpose of this lab was to extract DNA from human cheek cells. The isolated DNA could be used inmapping or sequencing‚ PCR‚ crime scene investigation
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Abstract This work describes the extraction of caffeine from tea leaves to determine its % caffeine. The extraction process selectively dissolves one or more compounds in a mixture into an appropriate solvent. In this experiment‚ it was visible in the process wherein the components of the tea leaves were dissolved in two solvents‚ water and dichloromethane (DCM)‚ with DCM used for multiple extractions. The organic layer was evaporated and the determined % caffeine was 0.12%. Furthermore‚ the purified
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lab‚ caproic acid was synthesized in a multi-step process that involved the synthesis of three intermediates – diethyl n-butylmalonate‚ potassium n-butylmalonate‚ and n-butyl malonic acid respectively. An IR was used to characterize the starting material‚ n-bromobutane‚ and the first intermediate‚ diethyl n-butylmalonate; while IR and NMR were used to characterize the final product‚ caproic acid. Reactions‚ Mechanism and Theory Caproic acid a.k.a n-hexanoic acid is a carboxylic acid derived from
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February 2000 is an American civilian official‚ Albert McFarland‚ it has been shock many citizens in Korea about this issue (Yang and U.S. Apologizes for Dumping Chemical). It leads to a complaint relationship between the United States and South Korea separation. Six years later‚ a monster is born from the Han River and attacks in the Riverside
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Separation of Sadness Sadness is a predator that can lurk in the minds of the emotionally injured. It can be inconspicuous and swift‚ or it can consume an individual and be the most prominent aspect of their life. It is complex and differs in severity. A person will generally feel three types of an emotional drag: a sort of nonchalant‚ short-lived sadness‚ a dwelling and despairing sadness‚ and an overwhelming sadness that takes over a person’s mind and being. Being upset is a temporary emotion that
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fermentation of ethanol results in the production of acetic acid (CH3COOH). The typical pH of vinegar ranges anywhere from 2 to 3.5‚ although shop-bought vinegar usually measures 2.4 www.wisegeek.com/what-is-vinegar.htm fig 1 In this study we will determine the amount of acid in a vinegar sample by using titration‚ a common technique in chemistry a typical shop brought vinegar has an acid percentage of 5% see fig 1. A titration is a technique where a solution of known concentration is used to determine
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coffee (by dry weight)‚ a typical serving contains much less‚ as tea is normally brewed much weaker. Besides strength of the brew‚ growing conditions‚ processing techniques- and other variables also affect caffeine content. Certain types of tea may contain somewhat more caffeine than other teas. II. Methodology Firstly‚ the extraction must be considered. Three tea bags were opened and the combined weights of the tea leaves were taken. Recording the weight is necessary. The tea leaves were returned
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reorganized the government of America into something entirely different from Britain’s monarchical reign. Additionally‚ the Americans started to implement new ideas and views on the social structure in America. Lastly‚ they had a distinct geographical separation‚ that demonstrated their individuality. By 1826‚ the United States is a truly legitimate‚ independent‚ and unique country‚ due to it’s radical structuralization of a nation. In the beginning‚ the Americans initially structured themselves under
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The framers of the Constitution created a political system based on limited government. The main point of this separation of powers was to limit any one branch from exercising the main functions of another; this starts in the division of government responsibilities into distinct branches. There are three distinctive branches‚ the legislative branch‚ the executive branch‚ and the judicial branch. Checks and balances came to play when the divisions of the branches were determined. With checks and balances
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Titration for Acetic Acid in Vinegar Purpose: The Primary Purpose of this Experiment is to Determine the Percent Content of Acetic Acid in a Household Bottle of President’s Choice Vinegar‚ using Titration Techniques. In addition‚ a Secondary Purpose for this Experiment that Derives Directly from the Primary Purpose is to Gain Hands On Experience in Titration Techniques‚ as a Vital Tool in our Quest to Understanding the Chemical Aspect of the World around Us. Procedure Conducting a Titration
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