THE EXTRACTION OF DNA THE EXTRACTION OF DNA 07/02/2013 | By Laura Green‚ Tasnim Rahman and Sandra Strammiello | Table of Contents DNA………………………………………………………………………………………..………… 2 The Discovery of DNA…………………………………………………………………………...…………..…………. 4 The Steps for Extracting DNA…………………………………………………………………………………………….…..… 5 Experiment: Which Product Can the Most Visible Amount of DNA Be Extracted From?..............................................................................................
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necessary reaction schemes. The reaction schemes should include molecular weights and any other important physical data for the reagents and products. Ex. Cyclohexanol was oxidized to cyclohexanone using sodium dichromate in the presence of sulfuric acid. * Note: you can download a free version of a structure editor from ACD labs‚ the PC version is called chemsketch‚ the Mac version is called marvinsketch. You should learn how to draw structures using these programs. Lab reports should look professional
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Extraction of R’-NH2 For both of the organic acid and organic base extraction‚ the addition of the respective inorganic acid or inorganic base (HCl or NaOH) to the three-component mixture caused an immiscible appearance of the solution in the separatory funnel. By briskly shaking the separatory funnel with the mixed components created a build up of pressure which was released through the stopcock forming small disappearing bubbles within the tip of the separatory funnel. Once the funnel was placed
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Unit 4 Assessment 2-Benzoic Acid Synthesis Synthesis and Investigation of Benzoic Acid Our aims: Create benzoic acid using benzaldehyde and hydrogen peroxide. Then remove some impurities from the benzoic acid crystals. Apparatus: Titration Pipette (25 cm3) Burette (50 cm3) Retort stand Clamp Conical flask (250 cm3) Volumetric flask (250 cm3) and stopper White tile Beakers (250 cm3) Dropping pipette Filter funnel Deionised water Phenolphthalein indicator Volumetric flask
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Recrystallization and Melting Points Determinantion of Benzoic Acid Abstract In experiment 2‚ recrystallization was used to purify the crude benzoic acid extracted from a mixture during experiment 1. Then a percent recovery for this recrystallization process was calculated. In experiment 3‚ the melting point ranges of the crude and purified benzoic acid were both determined by using Melt Temp Apparatus. Introduction Recrystallization is a purification technique for non-volatile solids. The general idea
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A. Title – Acid-Base Extraction with Separatory Funnel B. Introduction – The main objective of the experiment was the extract an acid‚ a base‚ and a neutral compound from a 2:1:1 compound of benzoic acid‚ p-nitroaniline‚ and azobenzene. In theory‚ an extraction technique based on phase distribution should allow two immiscible solvents to separate with a portion of the solute distributed into each. Using a separatory funnel‚ acids were extracted with bases (NaOH)‚ and bases were extracted
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Separation of Acidic‚ Basic and Neutral Substances by Extraction KEYWORDS Solubility‚ density‚ organic layer‚ aqueous layer‚ drying agent‚ protonation‚ recrystallization‚ neutralization‚ extraction ABSTRACT: The purpose of this experiment is to separate the substances in the acidic mixture and identify each constituent in the mixture that were individually separated using extraction. Three reaction tubes were used in the experiment. An acidic mixture containing benzoic acid‚ naphthalene‚
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Lab 5 Acid/Base Extractions Introduction The purpose of this experiment is to separate either the organic base (amine) or organic acid (carboxylic acid) from a mixture that contains inorganic impurities (salt) by performing a liquid-liquid extraction and then taking a melting point. Key Experimental Details and Observations Our starting material‚ Compound B‚ was a fine white powder and weighed 0.535g. The final product was a shiny white sheet that resembled acrylic paint and weighed 0.109g
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Separation by difference in particle size: Sieving: - Is a way pf separating mixtures of solids‚ or solids and liquids with different sized particles - The mixture is poured through a sieve and the particles smaller than the sieve pass through while the larger particles are trapped by the sieve - The are commonly used for cooking - E.g. a mixture of rice and water is poured through a sieve to capture rice and remove the water Filtration - Is a technique commonly used for mixtures of solids and liquid
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De-esterification for Synthesis of Benzoic Acid BATCH REACTOR Shane Bulk Chris Crosley David McGuire Max Skula Yunjing Song Shriram Sundarraj Nelson Zhou 155:416 Process Laboratory II Professor Jerry Sheinbeim January 28 – February 28‚ 2014 ABSTRACT The observed reaction that took place in this experiment was the de-esterification of ethyl benzoate to form benzoic acid. This experiment was used to determine the rate constant k of the synthesis of benzoic acid at different temperatures
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