Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
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Acids And BAses Acids And BAses 8.1 8.2 8.3 8.4 18.1 18.2 18.3 18.4 18.5 Theories of acids and bases Properties of acids and bases Strong and weak acids & bases The pH scale Calculations involving acids and bases (AHL) Buffer solutions (AHL) Salt hydrolysis (AHL) Acid-base titrations (AHL) Indicators (AHL) 8 8.1 THeORies OF Acids And BAses 8.1.1 Define acids and bases according to the Brønsted–Lowry and Lewis theories. 8.1.2 Deduce whether or not a species could act as a Brønsted–Lowry
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physical activity and obesity among urban‚ rural and slum populations in north India”. Obese 9(5):400-408. 5. Micha‚ R. and Mozaffarian‚ D. (2008)‚ “Trans fatty acids: effects on cardio metabolic health and implications for policy. prostaglandins‚ leukotrienes and essential fatty acids”‚ 79: 147-152. 6. Dhir‚ S. (2009) “Regulation of trans fatty acids in partially hydrogenated”. Food journal 109(2):182-195. 7. Center for Science in the Public Interest‚ (2012) “Trans fat: On the way out” Available at:
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Sam Chu (Jacob Gorman and Tyler Kui) Lab #1: Separation of a Mixture Mr. Mejia 10/3/14 Separation of a Mixture Lab Report Abstract The purpose of the experiment was to separate an initial heterogeneous mixture composed of 5.00 grams of salt‚ 2.00 grams of sand‚ 50.0 mL of water‚ 15.00 grams of pebbles‚ and 1.00 gram of iron filings‚ and leave as much salt as possible remaining. Using separation techniques including magnetizing‚ evaporation‚ filtration‚ etc. the heterogeneous mixture was thoroughly
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M. S. Ntamila and A. Hassanali University of Dares Salaam P.O. BOX 35061 Tanzania kolation of Oil of Clove and Separation of Eugenol and Acetyl Eugenol . An instructive experiment for beginning chemistry undergraduates chloride‚ and hydroxamic ester tests‚ and the preparation of the henzovl derivative of eupenol. Finally‚ ir spectra of the two components are examined‚ and the information ohtained comoared with that from chemical tests. Practical Detalls Crush about 30-35 g of cloves with pestle
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Extraction of Caffeine from Tea Leaves Marc Tugaoen‚ Kristine Vanzuela‚ Rafael Villanueva‚ Justeen Wong Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment has been divided into 4 set-ups‚ first was the solid-liquid extraction‚ next was the liquid-liquid extraction‚ then the sublimation and last was the melting point determination. The solid-liquid and liquid-liquid extraction were both done during the first meeting‚ the DCM layer was filtered and
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------------------------------------------------- Hydrochloric acid From Wikipedia‚ the free encyclopedia (Redirected from Muriatic acid) Not to be confused with hydrogen chloride. Hydrochloric acid | | | | Other names[hide] * Muriatic acid[1] * Spirits of salt[2] | Identifiers | CAS number | 7647-01-0 | ChemSpider | 10633809 | UNII | QTT17582CB | EC number | 231-595-7 | ChEMBL | CHEMBL1231821 | ATC code | A09AB03‚B05XA13 | Properties | Appearance | Colourless
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| Separation of Nitrophenols | | | | Abstract: Using a micro scale steam distillation we separated ortho and para-nitrophenol from a mixture that was already made. After the para and ortho were separated we measured their melting points and compared it to the literature values for purity. For ortho-nitrophenol we had 60% recovery and for para 160% recovery. Our melting point ranges were ortho: 45-46°C and para 64-95°C. Introduction: Nitration: In phenols‚ -OH group strongly
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Treatment of Results 1) What is the role of 0.25M sucrose as the medium for the fractionation process? Cold sucrose does not chemically react with cell organelles Due to the density and size of sucrose molecules‚ it is able to suspend pellets for configuration while providing a solution where the centrifugation can be better balanced Sucrose offers a liquid medium in which less dense fractions can be poured off as supernatant at the end of each centrifugation step. 0.25M sucrose solution
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Introduction to Organic Laboratoy Techniques 3rd Edition (2011) ** Students MUST obtain a copy of the textbook Synopsis This is an organic chemistry practical course which reinforce the theory and concepts studied in Organic Chemistry 1 (CHM456). It covers the learning of simple laboratory techniques such as reflux‚ distillation‚ extraction‚ crystallization and melting point determination‚ preparations of organic compound and separation of mixtures. The Techniques Students are REQUIRED to read
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