The Isolation‚ purification and identification of Proteins assayed From Bovine Liver Using SDS Gel Electrophoresis‚ Mass Spectroscopy and Western Blotting Abstract The purpose of the experiment was to isolate and recognize varying protein solubilization and assaying methods by using bovine liver protein. The experiment implicated the impact of different types of solvents like ethanol‚ water‚ PBS‚ PBS+1% Triton x-100‚ and PBS+2% SDS on protein solubilization. Bradford and Ghosh/Dumbroff
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Sahara and Ponzi schemes: What are the parallels? Dr KM Abraham‚ a former Whole-Time member of the Securities Exchange Board of India (Sebi) – the man whose original order led ultimately to a Supreme Court verdict forcing the Sahara Group to wind up two bond schemes and repay investors over Rs 24‚000 crore – made a brief allusion to Ponzi schemes in his order. While ordering two Sahara group companies‚ Sahara India Real Estate Corporation (SIREC) and Sahara Housing Investment Corporation (SHIC)
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TOPIC: Role of students in the purification of the society. TABLE OF CONTENTS TOPIC: Role of students in the purification of the society * Purpose of education. * Students and their role in the society. * Current situation of the society. * Students’ reformers in the past. * Students’ role in the purification of the society. * Conclusion
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-1- Voluntary Retirement Scheme (VRS) for the employees of Public Sector Undertakings. 1. SHORT TITLE: (i) (ii) (iii) 2. OBJECTIVE: (i) (ii) (iii) 3. This scheme may be called the Punjab State Public Sector Undertakings Voluntary Retirement Scheme (VRS) 2002. This scheme shall apply to all the Public Sector Undertakings (PSUs) including all Cooperative Institutions of the State of Punjab. This will apply to the Subsidiaries of the PSUs defined as entities in which PSUs
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Pyramid schemes are very harmful towards investors‚ and are hard to watch out for unless you know exactly what they are‚ why they fail‚ the effect of the failure‚ common victims‚ how to avoid them‚ and how to prevent them from spreading. More individuals are hurt than are helped when dealing with these scams. Individuals should be more informed about pyramid schemes‚ because they can be easily disguised. Participating in scams‚ will more than likely lead to a loss in money. “A pyramid scheme is a get-rich-quick
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A Ponzi scheme is a fraudulent investment operation that pays returns to its investors from their own money or the money paid by subsequent investors‚ rather than from profit earned by the individual or organization running the operation. Objectives We learn how it started. We learn the key elements in running a Ponzi scheme. We learn how big a Ponzi schemes can get. We learn how a Ponzi scheme falls apart. We learn how to identify and avoid being involved in a Ponzi scheme.
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VARIOUS SCHEMES IMPLEMENTED BY KVIC I. THE MARKET DEVELOPMENT ASSISTANCE (MDA) ON PRODUCTION SCHEME Khadi is handspun and hand woven cloth made by around a million traditional rural artisans. Handspun and hand woven characteristic is the unique selling proposition of Khadi. Govt. of India has been providing sales promotional assistance‚ popularly known as rebate on the retail sale value of khadi in order to facilitate the product to compete with other textile products in the market. The rebate
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perception on suggestion scheme. Introduction:- Suggestion scheme is a formalized mechanism which encourages employees to contribute constructive ideas for improving the organisation in which they work. The overall aim is to gather‚ analyse and implement ideas in order to create results that have a positive impact on the business and/or deliver new value to customers. There are two approaches to the suggestion scheme * Traditional approach-which
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Exercise 1: Bernard Madoff‘s Ponzi Scheme (i) Background of man involved (concern) in the case: Bernard Madoff was a former Chairman of the NASDAQ Stock Exchange‚ a Wall Street legend before his arrest. (ii) Ethical issues: In 2008‚ during the economic meltdown in the United States‚ Madoff could no longer honor his investor’s cash requests. When he admitted to this Ponzi scheme‚ it was a massive investment fraud that had affected many people including high profile investors. He pled guilty
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To purify the protein in the cell lysate from lab 1 through nickel affinity chromatography. Protein purification should result in only one type of protein ideally‚ which is the protein of interest‚ wt-DHFR and mut-DHFR in this case. A pure protein allows for further analysis on the protein to be conducted‚ such as its concentration (Bradford assay)‚ its molecular weight‚ and its biological activity. 2. Overview of experiments Buffer Preparation Add the liquid sodium phosphate‚ solid sodium chloride
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