1) Why serial dilutions are preferred to one large dilution in lab experiments? Answer: Serial dilution is the technique of performing repeated dilutions on the same chemical in order to change its concentration. The diluted solution from a serial dilution can be used to calculate the concentration of the actual solution. In experimental work‚ often we need to obtain a range of concentrations for a specific compound. Thus‚ instead of preparing one large dilution‚ if we take a concentrated sample
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DILUTIONS AND STANDARDS Many of the laboratory procedures involve the use of dilutions. It is important to understand the concept of dilutions‚ since they are a hand tool used throughout all areas of the clinical laboratory. These dilutions have to be considered as they make a quantitative difference in what is going on. First‚ there are several terms used in expressing dilution: 1. "Dilution: - Dilutions are expressed as the ratio of the quantity of a desired solute (serum‚ urine‚ chemical solution
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Vocabulary: Lesson pH and dilutions Science Starter: Determine the pH of .0103M H3PO4 solution in water. Remember to look at the H+ count. Box 1 In a dilution‚ the number of solute particles does not change‚ just the concentration with respect to the volume. This relationship is _____________________ proportional. Initially‚ there is 275mL 0.125M stock solution of a substance. -Determine the amount of moles of solute present. -What is the concentration after a dilution when the final volume is
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Serial Dilution Activity Many applications require the determination of microbial numbers. Those applications can be either clinical or in a research setting. Clinical applications include determination of antibiotic efficacy and as well as therapy. Research applications include determination of the effectiveness of antimicrobial chemicals‚ radiation‚ etc. The viable count is most common or standard method used to quantitate bacteria. With this method only microbes that are alive and able to reproduce
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First of all “A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration . Each dilution will reduce the concentration of bacteria by a specific amount” .( Study . com / Serial Dilution ) A serial dilution is any dilution were the concentration decrease by the same quantity in each successive step . The purpose of serial Dilution is to reduce the concentration of cells
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1. Introduction: The goal of this lab was to demonstrate the microbiology technique of serial dilutions and how they can effectively be used in experiments. E. coli cells were exposed to UV light for various amounts of time in order to asses the effect on growth and thereby mutations since this cell was modified to not have photolyse no uvr genes for DNA repair and thus can only use the SOS response. Each group then diluted the cells accordingly based on their UV exposure time and counted the cells
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OD was surveyed at 595. The UK protein in µg was assessed by taken the equation y=0.0259x+.0511 (Fig. 1) and solving for x where y is the OD. Calculation of UK Samp ID | µl of UK Assayed | OD @ 595 | µg UK protein | µg UK/µl(Diluted Sample) | Dilution Factor | UK Conc in µg/µl | 1:50 | 10 | .215 | 6.33 | .633 | 50 | 31.65 | 1:100 | 10 | .092 | 1.58 | .158 | 100 | 15.80 | 1:250 | 10 | .075 | 0.92 | .092 | 250 | 23.00 |
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available i. Spread plate method. ii. Most probable number (MPN) method. (I) SPREAD PLATE METHOD (LAWN CULTURE) MATERIALS: Dilution series prepared in (A) Sterile 1ml pipette 6 nutrient agar (NA) plates 1 glass hockey stick 1 beaker of alcohol PROCEDURE All steps should be done using aseptic technique The culture labeled 10-8 was gently mixed. 0.1ml of this dilution was aseptically transferred onto the center of a NA plate. Bend end of the glass spreader was dipped in alcohol‚ then it was
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Serial Murder The mind behind the crime! Thesis statement: Serial Murderers are not just murderers but also victims of the rotten hand they were dealt. Abstract I. Definition History A. The Most Infamous Killer B. Myth Theory 1. WereWolves 2. Vampires III. Causes A. Serial Killer Characteristics B. Theories VI. Case Studies A. Charles Manson 1. Bibliographical Info 2. Childhood Trauma B. John Wayne Gacy 1. Bibliographical Info
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Introduction Increasing more than ever‚ American society is fascinated with serial and mass murderers. Throughout the decades‚ people have celebrated killers who have reached the peak of success within their field by glorifying them in movies‚ documentaries‚ magazines‚ and even on trading cards. In 1991‚ a trading card company in California created its first mass and serial card collection‚ which included notorious murderers such as Jeffrey Dahmer who killed 17 men between 1978 and 1991. Dahmer
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