NON-INVASIVE PARAMETERS FOR THE DETECTION OF VARICEAL BLEED IN PATIENTS OF LIVER CIRRHOSIS‚ AN EXPERIENCE OF A TERTIARAY CARE HOSPITAL IN PAKISTAN Anam Umar (First author & corresponding author) Affiliation: Jinnah Postgraduate Medical Centre Email address: anam_umar@hotmail.com Mobile no: +923478300237 Fakhar Ali Qazi Affiliation: Jinnah Postgraduate Medical Centre Email address: dr_ar_lumhs@yahoo.com Mobile no: +923332724599 Rukhsana Abdul Sattar Affiliation: Jinnah Postgraduate
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quantitation is a study which is used to determine protein concentration in an unknown sample by comparing its assay response to one which is already known. This has uses in a broad range of research topics which include protein analysis. Two bovine serum albumin (BSA) dilutions of unknown concentrations using the Bradford assay method of protein quantification were used. BSA is a fatty acid carrier protein in the blood; this is used as it stabilizes enzymes and has advantageous properties such as protection
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Why was Mr. Lancaster so acidotic? (2 points) One reason why the patient was acidotic was because small intestine was not allowed to be perfused‚ causing cells to switch to anaerobic metabolism that produces lactic acid‚ thus decreasing serum pH levels. The patient’s HCO3 levels are near critical low levels 3. What is abdominal compartment syndrome? (4 points) ACS is a life threatening condition where increased abdominal pressure causes intraabdominal hypertension
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PATHOPHYSIOLOGY FOR THE HEALTH PROFESSIONS CHAPTER 6: FLUID‚ ELECTROLYTE‚ AND ACID-BASE IMBALANCES Fluid Imbalance Review of Fluid Imbalance -Water carries nutrients into cells and removes wastes‚ transports enzymes in digestive secretions‚ and moves blood cells around the body. Fluid Compartments -Approximatley 60% of an adult’s body weight consists of water -Infant’s body weight is about 70% -Fluid is distributed between the intracellular compartment (ICF)‚ or fluid inside the
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ring | * Acid hydrolysis is a better procedure than Alkaline hydrolysis because of the less destruction of amino acids Experiment 3: Protein Assay by Bradford Method * Coomassie Brilliant Blue G-250 * Bradford Reagent * Bovine Serum Albumin = BSA * Reagent blank = test tube #1 = 0mL BSA stock solution‚ 1mL distilled H2O * 595nm * Plot A595 vs concentration of BSA Experiment 4: Enzymatic Activity of Salivary Amylase * Iodine test – presence of starch * 1/t vs temperature/pH
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Clinical Chemistry 3 Lecture 5 CALCIUM HOMEOSTASIS AND HORMONAL REGULATION Roles of Calcium Signal transduction pathways àacts as a second messenger‚ in neurotransmitter release from neurons Contraction of all muscle cell types Fertilization Enzyme cofactor àblood-clotting cascade Proper bone formation PRINCIPAL ORGANS INVOLVED IN CALCIUM HOMEOSTASIS Small Intestines Bones (Skeletal System) Kidneys Normal Total Calcium: 2.2-2.6 mmol/L (9-10.5 mg/dL) Normal Ionized Calcium:
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PROTEIN ENERGY MALNUTRION Types include:[2] * Kwashiorkor (protein malnutrition predominant) * Marasmus (deficiency in both calorie and protein nutrition) * Marasmic Kwashiorkor (marked protein deficiency and marked calorie insufficiency signs present‚ sometimes referred to as the most severe form of malnutrition) Note that this may also be secondary to other conditions such as chronic renal disease[3] or cancer cachexia[4] in which protein energy wasting may occur. Protein-energy
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THE NURSING PROCESS: NURSING CARE PLAN NURSING DIAGNOSIS 2 (Problem; Etiology; Signs & Symptoms) P Decreased Cardiac Output R/T E Atrial Fibrillation and Mechanical Ventilation AEB S – Client on mechanical ventilation. Albumin 1.1 – 2/4/14 – low osmolality in blood – third spacing. Atrial Fibrilation Sluggish Pupil response Blood pressure 97/39 Heart Rate 54 Peripheral pulses diminished PLANNING ____________________________________________________________________________________
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Introduction Hemophilia is a rare genetic blood clotting disorder that primarily affects males. People living with hemophilia do not have enough of‚ or are missing‚ one of the blood clotting proteins naturally found in blood. Two of the most common forms of hemophilia are A and B. In persons with hemophilia A (also called classic hemophilia)‚ clotting factor VIII is not present in sufficient amounts or is absent. In persons with hemophilia B (also called Christmas disease)‚ clotting factor
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Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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