The primary antibody recognizes a specific amino-acid sequence of a particular protein. In this case‚ the antibody was anti-cow albumin. Then‚ it was incubated at 37°C for 30 minutes. After incubated‚ it was rinsed with TBS +NP 40 1x for 4 times. Each time was 5 minutes. This step was to wash the unbound antibodies. Then‚ it was rinsed two times with TBS 1x for 5 minutes to remove
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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were placed in micro-collection tubes containing potassium EDTA (Sarstedt‚ Inc.‚ Numbrecht‚ Germany). Blood for clinical chemistry evaluations was placed in tubes devoid of anticoagulant‚ allowed to clot at room temperature‚ and centrifuged‚ and serum was separated. Blood collected from all 10-mice/exposure groups was used for determination of each of the 16 haematology parameters. Adequate plasma samples obtained from another 10-mice/exposure group were available for determination of each of the
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ASSOCIATE DEGREE NURSING NURSING PROCESS FORM: PART I – ASSESSMENT Student: Date of Care: 3/4/13 Client’s Initial: WB Room # 1011 Occupation: Teacher Age: 59 Sex: F Race: Black Religion: Christian Admission Date: 3/1/13 Primary Language: English Role in family: Widowed from husband Stage in Life Cycle: Generativity vs. Stagnation Surgery date(s) this admission: N/A Chief complaint: Brain Dysfunction/Traumatic‚ closed injury Admission Diagnosis:
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Turmeric (Yu Jin) Curcuma longa (C. domestica) In This information about turmeric and how it is used as a herb in alternative herbal treatments to treat ailments and problems. It provides protection against cigarette smoke condensation‚ is used as an anti-cancer agent and to protect the cell’s DNA. | * Botanical classification of turmeric * Description of turmeric * Parts used * Properties of turmeric * Internal use * External use * Use of essential oil * Safety precautions
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Best Practice & Research Clinical Anaesthesiology 24 (2010) 365e374 Contents lists available at ScienceDirect Best Practice & Research Clinical Anaesthesiology journal homepage: www.elsevier.com/locate/bean 6 Neonatal fluid management Isabelle Murat‚ MD‚ PhD *‚ Alexis Humblot‚ MD‚ Laure Girault‚ MD‚ Federica Piana‚ MD Department of Anesthesia‚ Hôpital d’Enfants Armand Trousseau‚ 26 avenue du Dr Arnold Netter‚ 75571 Paris‚ Cedex 12‚ France Keywords: blood transfusion colloid
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References: Caifeng‚ N.‚ Zhang‚ Y.‚ Zhang‚ G.‚ Dong‚ C.‚ Shaomin‚ S. and Choi‚ M. F. (2009). Activation of nylon net and its application to a biosensor for determination of glucose in human serum. Enzyme and Microbial Technology 5‚ 249-253. Cengiz‚ E. and Tamborlane‚ W. V. (2009). A tale of two compartments: Interstitial versus blood glucose monitoring. Diabetes Technology and Therapeutics 11‚ 11-16. Chaplin‚ M. F. (2009). Biosensors. In molecular
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The result of a sodium/potassium electrolyte ratio of less than 25:1 along with increased levels of BUN‚ calcium and creatinine and a decrease in blood glucose and albumin concentrations may lead to a diagnosis of Addison’s disease. However‚ since electrolyte levels may not affect Addison’s disease an ACTH stimulation test provides a definitive diagnosis by testing the dog’s adrenal glands to produce cortisol. In this
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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