Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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to develop a standard curve for Bovine Serum Albumin‚ and this is done by preparing at least five dilutions. According to most research findings‚ scientists suggest that to determine the standard curve or concentration of an unknown sample of BSA using a quantitative spectrophotometer‚ the standard is diluted to a range from 0.01µg/ml to 10µg/ml. Material and method This particular experiment includes; spectrophotometer‚ 240µg/ml of Bovine Serum Albumin (BSA)‚ 5 test tubes‚ Coomassie Blue‚ beaker
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Diagnosis: 1. Lower limbs edema 2. Low albumin level 3. hypokalemia and hypocalcaemia 4. Anemia Nursing Diagnosis: Imbalanced nutrition: less than body requirements related to vomiting after eating as evidenced by food intake less than the recommended daily allowance and decreased albumin level. Name of client: Mrs. Tam Age: 65 Sex: Female Student ID:1155016494 Assessment date: 29/11/12 Medical Diagnosis: 1. Lower limbs edema 2. Low albumin level 3. hypokalemia and hypocalcaemia
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LABORATORY REPORT PRACTICAL 2 (ELECTROPHORESIS OF SERUM PROTEIN) DATE : 10 OCTOBER 2013 PART A SEPARATION OF SERUM PROTEINS USING THE ELECTROPHORESIS METHOD. OBJECTIVES: 1. To understand the analytical methods involved in analyzing serum proteins. 2. To study the serum protein electrophoresis pattern to aid the understanding of the relationship between the structure and the function of proteins. PRINCIPLE OF PRACTICAL: This technique is based on the movement of
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Recall that glucose is a monosaccharide‚ albumin is a protein with 607 amino acids‚ and the average molecular weight of a single amino acid is 135 g/mole. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer : c. glucose only Stop & Think Questions: The reason sodium chloride didn’t diffuse left to right is that You correctly answered: c. the membrane pore size was too small. Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average
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Facial Serums—The What‚ Why‚ and How These days it seems like some of the most unlikely concoctions end up being the most talked about beauty products and trends. I mean‚ we are living in a time where vampire facials (a.k.a blood facials) and sheep placenta facials are a thing. Doesn’t sound too appealing‚ does it? For those who are a little squeamish‚ it might be worth opting for tried and true methods and products—no blood or placenta involved! Today‚ we’re talking serums. A topical skin care
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Serum creatinine concentration shows a relationship with GFR‚ making it specific‚ but not a sensitive measure of GFR. The creatinine pool is influenced by nutritional state‚ hepatic disease gender‚ ethnicity‚ protein intake‚ and age (Slack et al.‚ 2010). In liver cirrhosis‚ the decrease in serum creatinine is due to a 50% reduction in hepatic production of creatine; increases in volume of distribution due to accumulation of extracellular fluid‚ ascites‚ edema‚ loss of muscle mass and malnutrition
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Introduction: The purpose of this experiment is to see the anti-cow antibody bind to cow serum only‚ and we expect to see the anti-cow antibody bind to the spot that had the cow serum. The system we use in this experiment is the serum from Cow‚ Horse‚ Goat‚ Sheep‚ and Donkey‚ Chicken. In order to able to detect and analyze proteins based on their ability to bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common
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requested: total protein‚ albumin‚ IgG quantitation‚ microbial culture‚ Gram stain‚ leukocyte count and differential cell count. The specimen should be sent to the various laboratories in which order? a. Chemistry lab‚ hematology lab‚ microbiology lab b. Hematology lab‚ chemistry lab‚ microbiology lab c. Microbiology lab‚ hematology lab‚ chemistry lab d. Hematology lab‚ microbiology lab‚ chemistry lab 3. You need to prepare a 1 to 4 (1:4 or ¼) dilution of a serum specimen using saline before
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grow strong and healthy lashes. Use an eyelash growth serum One of the simplest solutions is to go shopping and get an eyelash growth serum. It is recommended that you don’t choose a product randomly‚ due to the fact that some of them can affect your health. You should do some research before shopping for an eyelash growth serum‚ and make sure that you choose a product that has lots of good reviews from consumers. However‚ these sort of serums usually need to be applied for about a week or two for
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