when the gel electrophoresis is run. The Coomassie dye is brownish/red in color but turns blue when mixed with proteins. The blue color can be measured in a spectrophotometer at 595nm. A standard curve was generated using known amounts of bovine serum albumin (BSA). Four microfuge tubes were set up and 200ul of Bradford reagent were added to each tube and 1ul of whole cell extract was added to each appropriate tube. Entire sample of four tubes were transferred to four wells on 96-well microtiter plate
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Exercise 1: Cell Transport Mechanisms and Permeability Worksheet Student Name: Student ID# Student instructions: Follow the step-by-step instructions for this exercise found in your lab manual and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Rename this document to include your first and last name prior to submitting‚ e.g. Exercise1_JohnSmith.doc. Please make sure that your answers are typed in RED. Grading:
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we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to precipitate casein from skim milk and albumin from egg whites. The final product was weighed‚ and recorded. Final mass of albumin per egg was 0.142 grams. The final mass of casein from skim milk was 5.522 grams. These methods were both successful
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Chromatography (Greek for ‘colour writing’) is used to describe various methods applied to separate mixtures (referred to as the sample of the experiment) with great accuracy to analyze them. By using chromatography we can manipulate these to move at different speeds through the system‚ thus separating them. Chromatography is necessary in chemical industries‚ as well as bio processing companies. Chromatography can be: 1. analytical: used to measure ratios of analytes(substance in simpler forms)
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an end product and toxic product that should be efficiently eliminated from the body. As the bilirubin formed is water insoluble‚ it binds to albumin upon entering the blood as unconjugated or free bilirubin. Upon the bilirubin binding to albumins‚ it is transported to the liver‚ where it undergoes conjugation and excretion. Upon its separation from albumin on hepatocyte membrane‚ bilirubin binds to specific carriers and is intracellularly transferred to the site where it conjugates with glucuronic
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sodium chloride‚ glucose and albumin generated osmotic pressure. Which of the following would result in NO change in osmotic pressure across a membrane? The solutes can diffuse through the pores and the concentration of solutes is the same on both sides of the membrane. With the experimental conditions set at 10 mM glucose and 9 mM albumin‚ and the 200 MWCO membrane in place‚ which of the following is true? The net movement of water is toward the albumin. Which of the following can be true
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diffusion will occur between different concentrations. Material and Methods * Two beakers * Membrane holder * Four Dialysis membranes: 20 (MWCO)‚ 50 (MWCO)‚ 100 (MWCO)‚ 200 (MWCO). * Membrane barrier * Four solutes: NaCl‚ Urea‚ Albumin‚ Glucose * Solution dispenser * Deionized Water * Beaker Flush * Timer * Computerized Simulator Using the computerized simulator‚ the first step of the first experiment‚ you must place the 20 (MWCO)
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The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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1) Hypertonic refers to _______. 1) _______ A) the concentration of solutes that a cell is bathed in B) a concentration of solutes that is less than the solutes inside the cell C) the concentration of solutes inside a cell D) a solution that will cause a cell to swell 2) What happens to facilitated diffusion when the protein carriers become saturated? 2) _______ A) ATP will be utilized for transport. B)
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Medical Nutrition Therapy for Diabetes By Scott Roberts Introduction Diabetes is one of America’s most troubling medical issues‚ with over 23.6 million children and adults in the United States currently diagnosed with it. Food and lifestyle choices are fundamental to diabetes care. The role of the dietitian in the treatment of diabetes’ patients is crucial not only while in the hospital‚ but in the continued management after they are discharged. A thorough nutritional assessment should
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