an end product and toxic product that should be efficiently eliminated from the body. As the bilirubin formed is water insoluble‚ it binds to albumin upon entering the blood as unconjugated or free bilirubin. Upon the bilirubin binding to albumins‚ it is transported to the liver‚ where it undergoes conjugation and excretion. Upon its separation from albumin on hepatocyte membrane‚ bilirubin binds to specific carriers and is intracellularly transferred to the site where it conjugates with glucuronic
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sodium chloride‚ glucose and albumin generated osmotic pressure. Which of the following would result in NO change in osmotic pressure across a membrane? The solutes can diffuse through the pores and the concentration of solutes is the same on both sides of the membrane. With the experimental conditions set at 10 mM glucose and 9 mM albumin‚ and the 200 MWCO membrane in place‚ which of the following is true? The net movement of water is toward the albumin. Which of the following can be true
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when the gel electrophoresis is run. The Coomassie dye is brownish/red in color but turns blue when mixed with proteins. The blue color can be measured in a spectrophotometer at 595nm. A standard curve was generated using known amounts of bovine serum albumin (BSA). Four microfuge tubes were set up and 200ul of Bradford reagent were added to each tube and 1ul of whole cell extract was added to each appropriate tube. Entire sample of four tubes were transferred to four wells on 96-well microtiter plate
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diffusion will occur between different concentrations. Material and Methods * Two beakers * Membrane holder * Four Dialysis membranes: 20 (MWCO)‚ 50 (MWCO)‚ 100 (MWCO)‚ 200 (MWCO). * Membrane barrier * Four solutes: NaCl‚ Urea‚ Albumin‚ Glucose * Solution dispenser * Deionized Water * Beaker Flush * Timer * Computerized Simulator Using the computerized simulator‚ the first step of the first experiment‚ you must place the 20 (MWCO)
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1) Hypertonic refers to _______. 1) _______ A) the concentration of solutes that a cell is bathed in B) a concentration of solutes that is less than the solutes inside the cell C) the concentration of solutes inside a cell D) a solution that will cause a cell to swell 2) What happens to facilitated diffusion when the protein carriers become saturated? 2) _______ A) ATP will be utilized for transport. B)
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The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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FOUNDATION OF MEDICAL SCIENCE BIOLOGY-FGS0044 LAB REPORT 3.1 : CARBOHYDRATE DETECTION TEST LAB REPORT 3.2 : PROTEIN DETECTION TEST LAB REPORT 3.3 : LIPID DETECTION TEST GROUP MEMBERS : CONTENTS NO. | TITLE | PAGE | 1 | INTRODUCTION | 3 | 2 | LAB 3.1 : CARBOHYDRATES DETECTION TEST | 5 | 3 | LAB 3.2 : PROTEINS DETECTION TEST | 8 | 4 | LAB 3.3 : LIPIDS DETECTION TEST | 12 | 5 | REFERENCES | 14 | INTRODUCTION Carbohydrates (saccharides) are molecules consist of carbon
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Medical Nutrition Therapy for Diabetes By Scott Roberts Introduction Diabetes is one of America’s most troubling medical issues‚ with over 23.6 million children and adults in the United States currently diagnosed with it. Food and lifestyle choices are fundamental to diabetes care. The role of the dietitian in the treatment of diabetes’ patients is crucial not only while in the hospital‚ but in the continued management after they are discharged. A thorough nutritional assessment should
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School of Nursing & Allied Health Sciences SEMESTER: 2 INTAKE: JANUARY 2012 NBNS3504 COURSE: BACHELOR OF NURSING SCIENCE WITH HONOURS COURSE TITLE: RENAL NURSING MATRICULATION NO: 871001305396001 IDENTITY CARD NO. : 871001305396 TELEPHONE NO. : 0166695545 E-MAIL : annbukutty0110@gmail.com LEARNING CENTRE: PPW Melaka CONTENTS NO | TITLE | PAGES | 1 | INTRODUCTION * URINARY SYSTEM * WHAT DO NORMAL KIDNEYS DO? | 3 | 2 | RENAL REPLACEMENT THERAPY * HEAMODIALYSIS
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Brad is a 37 year old Caucasian male that entered the Emergency Department after experiencing a seizure in the middle of the night. The seizure was described by his wife as generalized‚ lasting approximately two minutes with tonic-clonic movements along with a loss of unconsciousness and function of his bladder. Brad reported that he has had headaches that have been unresponsive to over the counter medication over the last four month and his wife also notes that he has had an increased loss in energy
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