phenobarbitone (60mg/kg). The serum was separated by centrifugation at 10000 rpm for 10 min and analyzed for biochemical parameters such as serum urea‚ uric acid and electrolytes.Serum urea was measured using the commercially available kit (Liquicheck AGAPPE Diagnostics LTD)‚ following the GLDH-Urease method (Tietz‚ 1976). The amount of creatinine in serum was estimated using Liquicheck AGAPPE Diagnostics commercial kit according to Picrate method (Cook‚ 1975). Serum uric acid level was determined
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Topic: Stay Forever Young Serum Pattern: Criteria Satisfaction Purpose: After hearing this presentation‚ prospective customers will want to buy Morgan’s “Stay Forever Young” serum. Thesis: Stay Forever Young by Morgan is the best serum on the market to reduce all fine lines and wrinkles including dark spots and black circles. As Audrey Hepburn once said‚ “And the beauty of a woman‚ with passing years only grows!” (Hepburn) INTRODUCTION A. With some of the best ingredients in the
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fluid leaks from blood vessels into the pleural space. Chemically‚ transudate pleural effusions contain less protein and LDH (lactate dehydrogenase) than exudate pleural effusions. If both the pleural fluid–to–serum total protein ratio is less than or equal to 0.50 and the pleural fluid–to–serum LDH
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growth of these cells when treated with different calf serum and FGF concentrations. SRB protein assay was conducted in order to observe the cell proliferation. Western blotting was used to look at the relationship between the serum and activation of the Erk ½ pathway along with protein concentration. Thus‚ there were two hypotheses being tested during this experiment. This first hypothesis states: If various concentrations of Fetal Bovine Serum were added to non-transformed and transformed cells‚
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Ninhydrin‚ and Albumin solutions. If when we test each substance and the color changes‚ then that product has one or some of sugar‚ starch‚ protein‚ or lipids in it. Methods: As suggested in the book on page 36. Or otherwise instructed! Results: Food Product | Sugar | Starch | Protein | Lipid | Color change | Knowns | Benedict’s | Lugol’s | Ninhydrin | Sudan IV | | 5% sugar | + | __ | __ | __ | Blue to redish brown | 5% starch | + | + | __ | __ | Yellow to black | .1% Albumin | + | __ | + |
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the rate of diffusion. 5. Why do you think that the urea was not able to diffuse through the 20 MWCO membrane? 6. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. 7. Put the following in order from smallest to largest molecular weight: glucose‚ sodium chloride‚ albumin‚ and urea. ACTIVITY 2: Simulated Facilitated Diffusion Answers 8. True or False: The facilitated diffusion of glucose requires energy from ATP. 9. Which of the following
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samples were weighed; snap frozen on liquid nitrogen and stored at −70 °C to be processed for IL-1β and TNF-αdeterminations. Skin tissue was homogenized in phosphate buffered saline (PBS; pH=7.4) containing 0.4 M NaCl‚ 0.05% Tween-20‚ 0.5% bovine serum albumin‚ 0.1 mMphenylmethylsulfonyl fluoride‚ 0.1 mMbenzethonium chloride‚ aprotinin A 20 KI‚ and 10 mM EDTA. The homogenateswere centrifuged at 12‚000×g for 30 min at 4 °C‚ and then ELISA was used to measure the levels of IL-1β and TNF-α in the
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Ligaments.—The liver is connected to the under surface of the diaphragm and to the anterior wall of the abdomen by five ligaments; four of these—the falciform‚ the coronary‚ and the two lateral—are peritoneal folds; the fifth‚ the round ligament‚is a fibrous cord‚ the obliterated umbilical vein. The liver is also attached to the lesser curvature of the stomach by the hepatogastric and to the duodenum by the hepatoduodenal ligament (see page 1157). 17 The falciform ligament (ligamentum falciforme
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atoms may include oxygen‚ nitrogen‚ sulfur…etc. They can be found in nature‚ since “organic” means present in‚ or derived from‚ plants and animals. Materials/ Apparatus: Bunsen burner‚ 3 test tubes‚ test tube holder‚ dropper‚ red litmus paper‚ albumin (cystine)‚ sugar (sucrose)‚ urea‚ soda lime‚ 1% sodium hydroxide‚ hydrochloric acid‚ 5% lead acetate Procedure A: Testing for Carbon‚ Hydrogen and Oxygen 1. 1g of sugar (sucrose) was placed in a dry test tube. 2. The test
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3. Which MWCO dialysis membranes allowed both of these ions through? 50‚ 100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Na+/Cl-‚ Urea‚ Glucose 5. Which did not? Why? Albumin did not diffuse from the left to the right beaker. The conclusion is that Albumin is a polar substance. Polar substances have a much lower solubility in the membrane phospholipids. Certain compounds that are intermediates of metabolism are not usually allowed through the membrane‚ as
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