through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The system used in gel electrophoresis consists of 3 major parts: a stationary phase‚ a mobile phase) and an electrical power supply. In SDS-PAGE the polyacrylamide gel serves as the stationary phase
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Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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Jaundice (also known as icterus;[1] from the Greek word ίκτερος‚ attributive adjective: icteric) is a yellowish pigmentation of the skin‚ the conjunctival membranes over the sclerae (whites of the eyes)‚ and other mucous membranes caused by hyperbilirubinemia (increased levels of bilirubin in the blood).[2] This hyperbilirubinemia subsequently causes increased levels of bilirubin in the extracellular fluid. Concentration of bilirubin in blood plasma is normally below 1.2 mg/dL (50µmol/L) leads to
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TEXT BOOK OF BIOCHEMISTRY For 2nd year Medical Laboratory Technician Course (INTERMEDIATE VOCATIONAL) Author : S. Srinivasa Rao‚ B.Pharmacy‚ Jr. Lecturer in M.L.T.‚ Alluri Sri Rama Raju Govt. Junior College‚ Shanthi Nagar‚ Khammam. Editor : S. Kamalakar Rao‚ M. Pharmacy Senior Lecturer in Pharmacy‚ Department of Biotechnology‚ Govt. institute for post diploma courses in engineering and technology‚ Ramanthapur‚Hyderabad. INDEX Chapter I. Page No. Instrumental methods of Biochemical
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cellular recognition of certain signals ROLE OF THE CLINICAL CHEMISTRY LABORATORY: = most clin chem tests entail measuring the concentration of a particular constituent (the analyte) in body fluids‚ primarily blood plasma or serum = increased concentration could be caused by the ff: a.
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secretion of the anterior pituitary hormones. The nurse would expect the laboratory results to show a. increased urinary cortisol. b. decreased serum thyroxine. c. elevated serum aldosterone levels. d. low urinary catecholamines excretion. ANS: A Increased secretion of adrenocorticotropic hormone (ACTH) by the anterior pituitary gland will lead to an increase in serum and urinary cortisol levels. An increase‚ rather than a decrease‚ in thyroxine level would be expected with increased secretion of thyroid
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Kwashiorkor is a disease that is caused by malnutrition when there is not enough protein in the diet. Other names for Kwashiorkor are protein malnutrition‚ protein-calorie malnutrition‚ and malignant malnutrition (Kwashiorkor‚ 2011). Kwashiorkor is most common in areas where there is famine‚ limited food supply‚ and low levels of education. This disease can occur when people do not understand how to eat a proper diet. In very poor countries‚ this disease is more common. Often Kwashiorkor occurs during
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بسم الله الرحمن الرحيم Al-Quds University Body Fluid Lab. Report Chemical Examination of Urine Prepared By : Lucia Principles Benedict’s Test for Reducing Sugars Urinary sugars when boiled in Benedict’s reagent reduce copper sulphate to a reddish cuprous oxide precipitate in hot alkaline medium‚ the intensity of which is proportional to the amount of sugar present in the urine. The results are reported as 1+‚2+‚ etc. depending upon the colour and intensity of the cuprous oxide precipitate
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MTX Side Effects Applies to methotrexate: compounding powder‚ injectable powder for injection‚ injectable solution‚ oral tablet Hematologic Hematologic side effects have included myelosuppression which is one of the primary toxic effects of methotrexate. Methotrexate suppressed hematopoiesis has been reported to have caused anemia‚ aplastic anemia‚ pancytopenia‚ leukopenia‚ neutropenia‚ thrombocytopenia‚ lymphadenopathy‚ and lymphoproliferative disorders including reversible hypogammaglobulinemia
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Types of Hepatitis Lab test results show a positive anti-HCV and confirm that Chad has Hepatitis C. The nurse explains to Chad that Hepatitis is liver disease with inflammation of the cells of the liver. Chad states "I can’t have Hepatitis. I had all three of the vaccinations before I started working as a paramedic." There are five major types of viral Hepatitis: A‚ B‚ C‚ D‚ and E. 1. What information about the vaccination series for Hepatitis received by healthcare workers will the
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