thesis‚ we confirm the high-level expression of HIV-1 p24 antigen in transgenic tobacco plants through plastid transformation. PCR analysis confirmed the presence of the HIV-1 p24 sequence within the chloroplast genome of transgenic lines. SDS-PAGE gel electrophoresis of protein extracts from transgenic plants identified plant-expressed HIV-1 p24 protein. Quantification of the recombinant protein HIV-1 p24 using Aligent 2100 Bioanalyzer estimated yields of about 13 mg per g of soluble leaf protein
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Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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encoded fusion proteins was assessed by SDS-Page. Analyzing the gel under fluorescent conditions reveals protein bands which have the HaloTag® ligand attached. The PageRuler Plus prestained Protein ladder possesses two fluorescent bands‚ at 25 and 70 kDa respectively. HaloTag® Standard Protein with a size of 60 kDa was also analyzed and helps as an additional size reference. The HaloTag® features a size of 34 kDa alone. Agarose gel of PCR products after amplification of Campylobacter jejuni genes
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In unit one‚ we were introduced to the murder of Anna Garcia. We had to ‘visit’ the crime scene and document what we saw. In this unit‚ we learned many things‚ and conducted different experiments to help us understand what may have happened to Anna‚ and how she died. In this paper‚ you will read about what we learned‚ our theories about her death‚ and the evidence that helped us create our theories. The first piece of evidence we looked at was the fingerprints. We had prints from the victim‚ as
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largest eraser manufacturers in the world producing over 500 million pieces of erasers a year. In 1993‚ a new factory was built in Subang Jaya near Kuala Lumpur. Apart from being the first producer in Malaysia to manufacture writing instrument with GEL technology‚ this production facility also started manufacturing refillable markers and highlighters in 2010. Keeping up with the company’s philosophy of being innovative and competent‚ Faber-Castell Malaysia achieved many recognized certifications
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product range in L’Oreal hairstyle product range together with its most effective marketing strategy in the short and long run. Our Choice: Hair Wax According to ASEAN market trends data‚ it shows that market growth‚ particularly in hair wax and gel‚ increases significantly with a good projection value ahead. On average‚ the highest market growth in ASEAN is in hair styling agent with average of 6% market growth. Further supported by a research done by Mandom in their annual report 2012‚ ASEAN
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away with this price for 5.5 oz because of less competition close to that amount of oz. Also‚ it helps keeping the image of quality. Could also consider producing the 10oz at a higher price maybe at 6.95‚ which is the highest competitor price for a gel and that one had less ounces. Could justify by differentiation on quality and features. This option will be very profitable of priced like this. • Consider developing your own retail distribution methods so you can cut on rack jobbers variable costs
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Biocompatible nanomaterials are revolutionizing many aspects of preventive and therapeutic healthcare‚ which are already playing important roles in developing new medical devices‚ prostheses‚ bioassay technologies‚ tissue repair and replacement technologies‚ drug delivery systems‚ and diagnostic techniques. In recent years‚ much attention has been focused on the synthesis and applications of biocompatible functional nanomaterials‚ owing to their promising potential and practical applications
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organize those parts. Electrophoresis is the substrate electric movement of the separation of DNA. Gel Electrophoresis can separate both DNA and RNA. This can also allow the knowledge of the unknown bases of a strand of DNA. This can allow the knowledge of familiar patterns that can create a unique signature. Separation of these molecules
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typically the most laborious aspect of protein purification. The most abundant protein found in milk is caseins and it can be removed by heat and low pH. The most general method to monitor the purification process is by running a SDS-polyacrylamide gel electrophoresis of the different steps. This method only gives a rough measure of the amounts of different proteins in the mixture‚ and it is not able to distinguish between proteins with similar apparent molecular weight. the purest‚ isolated form
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