Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to
Premium Molecular biology DNA
Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
Premium Molecular biology DNA
CHAPTER – I INTRODUCTION Introduction to marketing: Every economic system has its own marketing system‚ in this competitive era and technological development requires a particular marketing system. In this word market there is a set up rules and regulations for exchanging goods and services. Marketing is an economic process by means of which goods and services are exchanged and their values determined in terms of money prices. It is that phase of business activity through which human wants are
Premium Marketing Pen
The identification of Bamboo using various PCR and Sequencing Techniques Abstract Often the incorrect bamboo species is sold to unsuspecting customers at shops. This can have a disastrous effect on their garden. Three separate and unknown Bamboo leaf samples were taken and were required to be distinguished genetically from one another. Using ITS-PCR DNA amplification techniques‚ the ITS region DNA was amplified and used in PCR-RFLP and RAPD PCR in order to determine the genetic identity of each
Premium Polymerase chain reaction DNA Molecular biology
Title: Feasibility of Calamansi Fruit (Citrofortunella microcarpa) As a Natural Anti-Dandruff Hair Gel Chapter I: Introduction Background of the Study: Rutaceae is a genus of plants with more than 160 species. It is also known as citrus family. Calamansi is one of the species of Rutaceae. The plant is characterized by wing-like appendages on the leaf stalks and white or purplish flowers. Its fruit has either a spongy or leathery rind with a juicy pulp that is divided into sections
Free Citrus
of new report “Global Industrial Silica Sand Market 2012 - 2016” in it’s database. To book your copy of this report @ http://www.industrymonitor.co/report/166461-global TechNavio’s analysts forecast the Global Industrial Silica Sand market to grow at a CAGR of 4.50 percent over the period 2012-2016. One of the key factors contributing to this market growth is the increased adoption of industrial silica sand for hydraulic fracturing. The Global Industrial Silica Sand market has also been witnessing
Premium Sand Process control Control theory
Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
Premium Chromatography Serum albumin
J Sol-Gel Sci Technol (2011) 59:73–94 DOI 10.1007/s10971-011-2465-0 ORIGINAL PAPER Sol–gel derived organic–inorganic hybrid materials: synthesis‚ characterizations and applications Sadanand Pandey • Shivani B. Mishra Received: 28 January 2011 / Accepted: 8 April 2011 / Published online: 22 April 2011 Ó Springer Science+Business Media‚ LLC 2011 Abstract Organic/inorganic hybrid materials prepared by the sol–gel approach have rapidly become a fascinating new field of research in materials
Premium Polymer Oxygen
INTRODUCTION Shaving gel is a viscous‚ clear gel preparation which is applied before shaving in order to soften the beard‚ lubricate the passage of razor over the face and moisturize the skin. Gels have cross-linked system which exhibits no flow in steady state. Desired characteristics of a Shaving Gel: * It should be clear and transparent. * It should have thick consistency but flowable. * It should lather easily when applied with brush. * It should give clean‚ fresh and moisturized
Premium Fatty acid
Proposal: How should our paper look like? (Total 14-15 pages) In the first part of the paper we will write an introduction and talk the approach we take to write the full paper. (1/2 page) In the second part of our paper we talk about ROIC/Payback period versus the NPV method of project valuations. The bottom-line is that NPV method has definite advantages over the other methods. (2-3 pages) In the third part we show that we will do the project valuation according to the NPV. We introduce
Premium Net present value