Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined
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CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
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thermal radiation. >Solar thermal energy conversion is implemented mostly in commercial and industrial applications and is encountered in two different types such are: a- Solar open-loop A/C; using air passing over solid desiccants (like silica gel or
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CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
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identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases are important in this method; one that is stationary and one that is moving. Chromatography works on the principle that
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Binding curves: CD can be used to monitor the binding if s substrate to a protein. The substrate can give a very different CD spectrum when free in the solution relative to when bond in solution. Outside of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography
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EXPERIMENT 8 THIN LAYER CHROMATOGRAPHY AND MELTING POINT DETERMINATION: DETECTION OF CAFFEINE IN VARIOUS SAMPLES Additional Resources http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html http://coffeefaq.com/caffaq.html Materials Needed TLC equipment: 1 5-cm x 8-cm TLC plate‚ 2 capillary micropipettes‚ TLC tank and lid‚ ruler‚ UV lamp Extraction Equipment: spatula‚ 2 small test tubes‚ Pasteur pipet and bulb Chemicals: caffeine‚ ethanol‚ dichloromethane‚ TLC solvent (5% acetic acid in ethyl acetate)
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Bayer process is the principal industrial means of refining bauxite to produce alumina (aluminium oxide). The aluminium oxide must be purified before it can be refined to aluminium metal. Important Ore: BAUXITE. CONTENTS: Al2O3 - 30 -54% Rest -Silica‚ Iron Oxides and Titanium Oxides. ADVANTAGES Most economic means of obtaining alumina from bauxite. APPLICATIONS industrial and medical ceramics sandpapers pigments cosmetics Pharmaceuticals. FLOW CHART: THE PROCESS STAGES
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LAB REPORT ANDREW O. BAAFI Organic Chemistry I Lab. Instructor: Dr. Strange 09-24-01 TITLE: Preparation of Isopentyl Acetate (isoamyl acetate) / Gas Chromatography (Esterification). REFERENCE: Fischer‚ W. H.‚ A. G. Craig. “Microscale Esterification of Peptides and Analysis by MALDI-MS.” http://www.salk.edu/LABS/pbl/brukeran1.htm. 10-02-2001 Mayo‚ Dana W‚ Pike Ronald M‚ Trumper Peter K. Microscale Organic Laboratory. 3rd ed. New York: Wiley & Sons‚ 1994. Strange De Soria‚ Louise. Chemistry
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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