progress of the extraction. Thin Layer Chromatography (TLC) Plating: This is a common method that is used to monitor the progress of a reaction and determine when it is complete. In our chromatography there was a stationary phase/adsorbant of Silica gel and we used mobile phases of ethyl acetate‚ hexanes‚ and a 2:8 mix of both. During the process‚ the mobile phase passes over the compound spots at the bottom and drags them up the adsorbant. The resulting spots where the compounds stop can be used
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test solution and dilute to 100.0 ml with the mobile phase. D. N‚S-diacetyl-L-cysteine. The chromatographic procedure may be carried out using : — a stainless steel column 0.25 m long and 4.6 mm in internal diameter packed with octadecylsilyl silica gel for 01/2005:0309 chromatography R (5 µm)‚ — as mobile phase at a flow rate of 1 ml/min a mixture ACETYLSALICYLIC ACID of 2 volumes of phosphoric acid R‚ 400 volumes of acetonitrile for chromatography R and 600 volumes of Acidum acetylsalicylicum
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mobile phase and the stationary phase. The mobile phase must be degassed to eliminate the formation of air bubbles. Types: Partition chromatography Adsorption‚ or liquid-solid Liquid chromatography Ion exchange chromatography Size exclusion‚ or gel‚ chromatography Bioaffinity chromatography Absorption chromatography Composition: Solvent Solvent Delivery System (Pump) Injector Sample Column Detectors (Diode Array) Waste Collector Recorder (Data Collection) Mechanism
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S OUTHEAST ASIAN J T ROP MED P UBLIC H EALTH ANTI-HEAD LICE EFFECT OF ANNONA SQUAMOSA SEEDS Junya Intaranongpai 1‚ Warinthorn Chavasiri 2 and Wandee Gritsanapan 1 1 Department of Pharmacognosy‚ Faculty of Pharmacy‚ Mahidol University‚ Bangkok; Department of Chemistry‚ Faculty of Science‚ Chulalongkorn University‚ Bangkok‚ Thailand 2 Abstract. The present study focused on the separation and identification of the active compounds against head lice from the hexane extract of Annona squamosa
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availability of seeds for future uses. Clawford stated that in the absence of mechanical scarification‚ seeds that are dormant remains "impermeable" yet still viable‚ this characteristics is an ideal in the seed atorage processes. Dessicants‚ like silica gel‚ sawdust‚ ash‚ and lime also helps in the maintenance of moisture that is critical in seed storage. This exercise is done in the need of determining the relationship between seed germination‚ seed dormancy and storage. It is also aimed in determining
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hr heating both the boiler and the distillation flask. The receiver contains oil of clove‚ some separated and some dispersed in water. Extract the mixture with 3 portions of 25 ml chloroform. Examine the chloroform extract using the tlc plates (Silica Gel coated plates and running with chloroformlligroin: Y4). Develop the plate by exposing to iodine vapor or preferably spraying with concentrated sulfuric acid in ethanol and then heating a t 120°C. Retain a small sample (0.5 ml) of the chloroform
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Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also
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Objective The purpose of this experiment is to identify an unknown proprietary drug using thin-layer chromatography. The unknown’s behavior in thin-layer chromatography will be compared with that of its possible component analgesics. The possible unknowns and their analgesic ingredients will be Anacin (aspirin‚ caffeine)‚ Excedrin (acetaminophen‚ caffeine‚ aspirin)‚ Motrin (ibuprofen)‚ and Tylenol (acetaminophen). Introduction Thin-layer Chromatography(TLC) was the method used to figure out and
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HIGH TECHNOLOGY FIBRES FOR TECHNICAL TEXTILES 1 INTRODUCTION Human life is surrounded by hundreds of textile fibres either in the form of clothes‚ interior textiles or in the form of high performance technical textiles made of conventional or high technology fibres for various applications. A textile fibre is usually defined as a flexible‚ macroscopically homogenous cylindrical body mainly with circular cross- section having a high ratio of length to diameter (typically 100-3000: 1). Textile
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Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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