SYNTHESIS OF STRONTIUM HEXAFERRITE SUBSTITUTED WITH TITANIUM AND ZINC NANOPARTICLES BY SOL-GEL METHOD AND ITS CHARACTERIZATION BY PAVITRA THIRUCHELVAM SCHOOL OF ARTS AND SCIENCE TUNKU ABDUL RAHMAN COLLEGE KUALA LUMPUR 2012/2013 SYNTHESIS OF STRONTIUM HEXAFERRITE SUBSTITUTED WITH TITANIUM AND ZINC NANOPARTICLES BY SOL-GEL METHOD AND ITS CHARACTERIZATION BY PAVITRA THIRUCHELVAM A project report submitted to the School of Arts and Science in partial fulfilment of the requirement for the Bachelor
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Christian Hogdohm Gel Electrophoresis I. Introduction: A typical electrophoresis has five major parts: the electrical current‚ DNA‚ RNA‚ or protein sample‚
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Objectives Protein Isolation: Protein isolation for a western blot uses detergents and mechanical force to separate seeded cells from their container. Eukaryotic cells are attached to the surface of a flask by cadherins. In the past‚ we’ve separated the cells from the flask by breaking these bonds with a protease‚ but in order to keep the proteins intact‚ a different method needs to be used to extract the proteins. In protein extraction for a western blot‚ we use detergents to lyse the membrane
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bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common technique used to separate proteins based on molecular weight under the influence of an applied electrical field and then used to prepare for the Western Blot (#1 Lehninger). The support medium used is a polyacrylamide gel and‚ it also used sodium
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and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according to size using an electric current. Proteins that are separated after are blotted from the gel and onto a paper membrane. An antibody is then added to the membrane paper and causes a colored reaction. Following the reaction‚ the results help detect and quantify a single
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separation of macromolecules in an electric field is called electrophoresis. A very common method for separating proteins by electrophoresis uses a discontinuous polyacrylamide gel as a support medium and sodium dodecyl sulfate (SDS) to denature the proteins. The method is called sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) where one can obtain information about the size of a protein or its molecular weight and yield or the total amount of the protein. This system is also
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was designed to use western blot analysis to detect proteins from Non-fat powdered milk. The experiment had to be run two times before the expected outcome was reached. However‚ after the second attempt‚ it was successful. The gel ran well‚ and the proteins from the gel were successfully transferred to the membrane provided in the kit. Introduction The name western blot was given to the technique by W. Neal Burnette and is a pun on the name Southern blot. Southern blot was a name given by
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the boxes below. Enter the data (number of bands‚ description of the band patterns) you collected for the protein ladder in the appropriate columns for the gel concentrations (8%‚ 10%‚ 12%) | | | | | |Gel |8% |10% |12% | |
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
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