bacteria do not maintain the crystal violet dye used in the gram staining method because of their cell walls that are thin .most of the gram negative bacteria are usually harmful to the host. (Gram negative rod shape salmonella entrica) There are several tests and experiments that classified the genus of the unknown species‚ which known as gram staining method. Carbohydrate fermentation: 1) Phenol red lactose broth
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
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The problem that was required to be investigated in this laboratory experiment was to determine which type of cleaning product would be able to prevent the most bacterial growth when placed in a neutral environment. In order to answer this problem‚ 8 different disinfectants were tested‚ including sterile water‚ and their after two days their zones of inhibition were measured and recorded. A hypothesis that was formulated before experimenting was‚ “If the type of disinfectant is Mouthwash‚ then it
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Agar What is it and where is it from? Derived from the cell walls of red algae‚ agar is a gelatinous substance extracted through a process of boiling and filtration. Agar is specifically used in laboratories for diagnostics and experimental purposes. Agars main purpose is to act as a growth medium for micro-organisms such as bacteria and fungi. The micro-organisms feed off the nutrients contained in Agar and can be both cultured and observed within it by scientists and the use of a microscope. Using
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D4 H and E staining procedures Haematoxylin and eosin staining is a technique used by pathologists to add definition to nuclei and other parts of a cell in tissue samples under a microscope so that they can see it better when analysing the cells form and abnormalities. The H&E technique is the most commonly used in histology and the diagnoses of malignancies is based largely on the results of this procedure. The dyes will specifically stain elements of a cell or tissue because the dyes have a high
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below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive but your gram stain results indicated that it was gram negative‚ how would you proceed and can you think of any reasons why this may
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distinguishes between Gram-positive and Gram-negative bacteria based on the composition of the cell walls. Gram-positive bacteria appear purple and Gramnegative bacteria appear pink after staining. The first Gram stain produced unsatisfactory results and was then repeated with a clear indication of negativity. Light pink staining was evident on the cells in the field of view (FOV) and a search of the slide revealed uniformity in the sample. From these results we
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Effectiveness of Basella rubra (Alugbati) Fruit Extract as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists
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Question 1 of 12 2.0 Points All of the following are considered microbes except A.viruses B.bacteria C.protozoa Correct D.worms Answer Key: D Question 2 of 12 2.0 Points Microbiologists investigate problems by designing and carrying out experiments. What is true about the scientific method? A.a hypothesis is the definitive explanation to account for the observation and therefore does not need to be tested B.a prediction is the factor that can change
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