Abstract: A patient arrived at the hospital recently and brought a sample their infectious disease on an agar plate. A series of tests were completed in order to identify this unknown sample. First‚ the plate was analyzed for its hemolysis characteristics. Afterwards‚ a Gram stain was performed from a sample of the agar plate and the slide was viewed under the microscope. Once‚ the microscopic visual was captured‚ a catalase test followed. Next‚ for further data‚ MSA results were recorded‚ along
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#3. Name the types of microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside
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MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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of the unknown bacterium were taken after 48 hours of incubation at 35° Celsius. Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure
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most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium. Results:
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the bacteria first. The stain serves to define the bacterial cells. All bacteria are either gram negative or gram positive. To stain a sample first the scientist has to “set” the sample. Setting the sample is the basis for all stains. To “set” or simple-stain a sample of
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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classified on the basis of many characteristics. Morphological and physiological features such as cell shape‚ motility‚ formation of spores and other distinguishable structures‚ and reaction to Gram stain is a good start in identifying bacteria. Other staining techniques such as Acid Fast stain are also useful in determining species. More important in identification of a genus and species of bacteria are biological tests‚ including the determination of the types of nutrients a cell can use‚ the products
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inoculation‚ we were able to visibly see the various bacterial colonies of our biofilms. We proceeded to gram-stain a colony from each biofilm‚ in search of colonies of gram-positive bacteria. There are very specific steps that must be taken when gram- staining bacteria; the first step being to collect a colony of bacteria from one of the TSA plates‚ then transfer the colony from the TSA plate to the slide. We used a sterile loop (sterilized by placing the loop in the flame of a Bunsen burner to eliminate
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this paper will be on the description of the just one of the three bacteria and some of its effects on humans. The first test done was a differential stain (gram stain) to determine the unknown microorganism cell shape and arrangement. The gram staining is done by first putting a sample of the unknown bacteria on a slide and it was heat fixed taking care not to over or under fixed the slide. The slide was flooded with crystal violet and let stand for 30 seconds and then rinsed with water. Secondly
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