correctly and be able to see it under the light microscope. In 1883 Hans Christian Gram discovered an important staining method that is used extensively today. The stain is called the Gram Stain. This experiment was done in order to differentiate microbes into two basic groups: Gram positive microbes and Gram negative microbes. The purpose of this experiment was to learn the gram staining method and to observe the characteristics of gram negative and gram positive microorganisms. Materials: Microscope
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As mentioned above‚ bacterial growth rates during the phase of exponential growth‚ under standard nutritional conditions (culture medium‚ temperature‚ pH‚ etc.)‚ define the bacterium’s generation time. Generation times for bacteria vary from about 12 minutes to 24 hours or more. The generation time for E. coli in the laboratory is 15-20 minutes‚ but in the intestinal tract‚ the coliform’s generation time is estimated to be 12-24 hours. For most known bacteria that can be cultured‚ generation times
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Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent
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slide before staining? Differentiate between a differential stain and a special stain (how are they different‚ don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure
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Activities/Tasks/Responsibilities | Problem(s) Encountered | Action(s) Taken | Personal Reflection | BacteriologyJuly 4- July 31 | Receiving of specimen‚ Logging‚ Processing of specimen‚ Inoculation into media‚ biochemical testing‚ identification of organism‚ gram staining‚ AFB staining‚ releasing of results‚ culture sensitivity testing. | No major problems were encountered during my rotation for Bacteriology. Minor problems like unfamiliarization of SOP’s and other procedures are encountered. | Asking proper procedures
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Bunsen burner‚ glass slides‚ lens paper‚ bibulous paper‚ regular tap water‚ along with reagents Crystal violet‚ Gram’s Iodine‚ 95% ethyl alcohol‚ and safranin. The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this‚ I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable end through the flame‚
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Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial colony was isolated form Mycorrhizal spores‚ and further tests done on that colony. Sub culturing was done after each week to ensure
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different colonies were observed‚ the first colony was yellow in color and larger in size and the white colored colony was slightly smaller in size. As instructed‚ each colony was prepared for gram staining‚ one slide for the large yellow colony and one for the smaller white colony. After properly gram staining the slides as directed in chapter six of the lab manual‚ the smears were examined
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Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories of bacteria. Gram positive cells have an extra
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multiple types of testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe
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