| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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3.1. Isolation and identification of Bacterial isolates An enrichment culture technique was used for the isolation of bacteria responsible for biodegradation of phorate in soil. Screening of these bacterial species for phorate degradation in liquid cultures in our previous study (Jariyal et al.‚ 2014)‚ resulted in identification of bacterial species B. aerophilus strain Imbl 4.1 ‚ Brevibacterium frigoritolerans strain Imbl 2.1 and Pseudomonas fulva strain Imbl 5.1. However‚ these bacterial species
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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1. Make sure you label both plates on the bottom‚ with your name‚ date and name of pathogen you have chosen (listed above under materials) Then label numbers 1-5 on one plate and 6-10 on the other as there are 10 disks that need to be placed. Spread out in a circle formation like pictured below (This is the final product; look at this for reference on how to label) Now you are ready to start culturing. Start with one of your plates you can do both at the same time but to avoid making any errors
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Lakeli is absolutely stunning‚ but for his beauty he has paid the price. He is a beautiful 12 week old deaf Great Dane. While we all think white Great Danes are stunning most of them are going to be either blind‚ deaf‚ and occasionally both. What is a Double Merle and how are they created? A few things to keep in mind about the Merle pattern as you read. •Merle is a pattern not a color. •Merle is a bleaching pattern. •The Merle gene creates mottled patches of color in a solid color coat‚
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The tube that contained my unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative.
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1. Title: Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria
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“The simple gift” written by Steven Herrick is narrated in the first person in helps to gives a sense of subjective‚ since it has the character’s own prejudices and preferences such as “I knew it was hunger”. It engages the reader by building up the closeness with them‚ and allows them to delve into the mind of the characters. “The simple gift” is also written in a diary format as from the three different characters points of view‚ in affect to be more personal and sincerely. This is the main technique
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Start a Simple Budget and Plan For Retirement By Jack Landry Sep 15‚ 2011 A simple budget can help you reach your financial goals sooner with less stress and worry. A budget is a plan you put together to track the flow of money into and out of your life. The idea is to find where your money flows out‚ plug the expensive leaks‚ and save more inflowing cash for yourself every month. A basic budget can also expose the sneaky spending habits you’re not aware of and help you plan where to best spend
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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