Unknown Experiment Introduction: The purpose of this experiment is to distinguish and indentify an unknown bacterium. There are several tests that can help one eliminate and narrow down the options. The most useful test‚ and the very first one done‚ is a gram stain. This test will tell whether the bacterium is gram-positive or gram-negative. After the type of gram stain is identified‚ the tester has a wide array of differentiating tests at their disposal. Based on the results from these
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Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials
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I was able to come to the conclusion that my unknown is Klebsiella pneumoniae through the use of Bergey’s Manual. After performing a gram stain of my unknown I was able to determine that my unknown was a gram-negative rod. While testing the oxygen requirements my test tube had growth throughout the tube‚ indicating my unknown was a facultative anaerobe‚ which can grow in the presence or absence of oxygen. The most important test was whether my unknown was motile or nonmotile. My test tube only showed
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Lab Report: Effects of Lysozyme Introduction: This report discusses an experiment that was done to demonstrate the effects of lysozyme on populations of Gram positive and Gram negative bacteria. Bacteria have a cell wall composed of peptidoglycan that gives the wall its strength. Gram negative bacteria have and extra component of lipopolysaccharide (LPS)‚ that is stabilized with magnesium ions‚ to their cell wall that further protects them. When Gram positive bacteria are treated with lysozyme
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The purpose of this exercise was to use our skills we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
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CHAPTER I INTRODUCTION 1.1 Background Sentence‚ either it is simple or complex sentences‚ are always containaining at least one verb. Sentence is now lately called clause. Futhermore‚ a clause that consits of one verb is called simple clause and a clause that consits of more than one verb is called complex clause. To complete the meaning of a sentence‚ the elements object (consists of noun or noun pharase)‚ complement‚ and adjunct (place) should be added because some verbs needs complementations
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instructed to be invested at 10% p.a. simple interest. How much did the elder daughter get at the time of the will? (1) | Rs. 17.5 lakhs | | (2) | Rs. 21 lakhs | | (3) | Rs. 15 lakhs | | (4) | Rs. 20 lakhs | Correct Answer - (2) Solution: Let Rs.x be the amount that the elder daughter got at the time of the will. Therefore‚ the younger daughter got (3‚500‚000 - x). The elder daughter’s money earns interest for (21 - 16) = 5 years @ 10% p.a simple interest The younger daughter’s money
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EXPERIMENT 7 - Distillation – Separation of a Mixture Purpose: a) To purify a compound by separating it from a non-volatile or less-volatile material. b) To separate a mixture of two miscible liquids (liquids that mix in all proportions) with different boiling points. Equipment / Materials: large test tubes(3) clamp (1 or 2) ringstand boiling chips test tube rack (1) heating mantle glass adaptor thermometer adaptor 10-mL graduated cylinder condenser (1 or 2) grease 50- mL round bottom flask 50-
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