and therefore necessary during cellular proliferation. The protein can be found in all phases of the cell cycle but not in resting cells. The Ki67 staining is used to determine growth of certain cell types and is therefore used on hyperproliferative cells such as tumor cells‚ but also in benign hyperproliferative diseases such as psoriasis. The staining reveals the proliferative active keratinocytes which can be found in stratum basale. Sept7-deficient mice receiving IMQ treatment (Fig S6. A) had
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Week 1 Chapter 3 Staining Lab Quiz SCI/250 SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each) Check or underline the correct answer Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall
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PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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employee a number of approached to acquiring a pure culture from a from sample containing a number of different types of bacteria. Briefly describe three different procedures commonly used to secure pure cultures from a mixed culture. The use of simple labeled diagrams may be quite helpful. 4. Microbiological media exists in a variety of forms and are classified on the basis of certain criteria. a. Describe the various physical states into which media are commonly classified
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endospores of living bacilli appear edged in black and are very bright and refractile. Endospores strongly resist application of simple stains or dyes and hence appear as nonstaining entities in Gram-stain preparations. However‚ once stained‚ endospores are quite resistant to decolorization. This is the basis of several spore stains such as the Schaeffer-Fulton staining method which also differentiates the spores from sporangia and vegetative cells. However this method is not suitable for taxanomic
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microbiology is the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke‚ the English biologist who observed algae and fungi in the 1660s. In the 1670s‚ “Anton van Leeuwenhoek‚ a Dutch merchant‚ constructed a number of simple microscopes and observed details of numerous forms of protozoa‚ fungi‚ and bacteria” (Introduction to Microscopes‚ n.d.). During the 1700s‚ microscopes were used to further explore on the microbial world‚ and by the late 1800s‚ the light microscope
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prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria‚ only 10% cause disease. The three (3) diseases and their causative
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tuberculosis distinguishable from nearly all other bacteria. Acid-fast staining is‚ therefore‚ known as a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized by a powerful solvent known as acid alcohol. Most other bacterial genera are easily decolorized by acid alcohol. Ziehl/Neelsen Acid-Fast Staining Procedure In later years‚ Ehrlich’s technique was improved upon
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Theories of Staining Techniques Staining bacteria with different dyes via staining techniques‚ allows in distinguishing the microorganism from its backgrounds. Also‚ helps in studying different internal structures such as vacuoles‚ cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining‚ endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also‚ these staining procedures help in determining properties
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image. Studyblue.com. STUDYBLUE INC.‚ 22 Apr. 2013. Web. 3 Nov. 2013. Reynolds‚ Jackie. Selective and differential media. Digital image. Www.microbelibrary.org). Kern Community College District‚ 12 Sept. 2011. Web. 3 Nov. 2013. Rose‚ Scott. B. cereus simple stain. Digital image. Napa Valley College. The Western Association of Schools and Colleges Designated Hispanic Serving Institution (HSI)‚ US Department of Education‚ n.d. Web. 5 Nov. 2013. Rose‚ Scott. Gram Stain B. cereus. Digital image. Napa Valley
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