Direct competitive cocaine immunoassay was performed in a transparent high binding 96 well microtiter plate. Each well was coated with 200 µL of 1mg/L anti-mouse IgG in PBS (phosphate buffered saline‚ 10 mM sodium dihydrogen phosphate‚ 70 mM sodium hydrogen phosphate‚ 145 mM sodium chloride‚ pH 7.6). The microtiter plate was left over night for shaking at 750 rpm. Therefore‚ the plate was washed three times with automatic 96-channel plate washer by washing buffer (45 M potassium dihydrogen orthophosphate
Premium Water Chemistry Sodium chloride
Nitrocellulose membrane filters Silicon powder Wetted explosives Sulfur Titanium Powder‚ wetted Zinc resinate Naphthalene Spontaneously Combustible Activated carbon Lithium alkyds Pentaborane Phosphorus Potassium sulfide‚ anhydrous Oily rags Seed cake Sodium sulfide‚ anhydrous Butyl lythium Dangerous When Wet Alkaline earth metal alloys
Premium Metal Calcium Liquid
Substitution reaction of Molybdenum Hexacarbonyl: the use of infrared spectroscopy as a structural tool in metal carbonyl chemistry. Abstract The synthesis of isomer A and B of [Mo(CO)4(PPh3)2] were prepared from molybdenum hexacarbonyl‚ sodium borohydride and triphenylphosphine with a % yield of 79% and 8.5% respectively. Two isomer was identified using IR spectra and from isomer A four C≡O stretching was observed‚ while in isomer B only one C≡O stretching was observed in a frequency ranged
Premium Chemistry Chemical reaction Hydrogen
UNIVERSITI TEKNOLOGI MARA COURSE INFORMATION Confidential Code Course Level Credit Hours Contact Hours : : : : : CHM 556 Organic Chemistry II Degree 4 3 hr (Lecture) 3 hr (Practical) 3 Core CHM 456 Part Course Status Pre-requisite : : : Course Outcomes : Upon completion of this course‚ students should be able to: 1. Determine functional groups present in organic compounds using Infrared Spectroscopy and interpret Nuclear Magnetic Resonance spectra and relate the information to structural
Premium Carbonyl Ester Carboxylic acid
substitution reactions. In this experiment two geometric [Mo(CO)4(PPh3)2] isomers A and B were synthesized and their molecular geometries were determined by means of infrared spectroscopy. Isomer A was synthesized first by reacting [Mo(CO)6] with sodium borohydride employed as a reducing agent and triphenylphosphine. A satisfactory 90 % yield was obtained for this isomer. From Isomer A produced‚ 0.502 g was taken and then used to synthesize Isomer B which was obtained at a yield of 50 %. The infrared spectra
Premium Infrared Infrared spectroscopy
Preparation In a dry round bottom flask‚ 1.5mL of cyclohexanone was added with 5mL of methanol. The solution was cooled in an ice bath for 3 minutes after which 0.2g of sodium borohydride was measured and added to the solution. Upon mixing‚ a gas was formed (bubbles). The round bottom flask was removed from the ice bath and placed at room temperature. After 10 minutes at room temperature‚ 5.0mL of 3M NaOH and 2.0mL of distilled water was added to the solution. Isolation and Characterization The
Premium Water Oxygen Distillation
Using Selective Media to Monitor the Fermentation Process of Cabbage and Cucumber‚ for Fourteen Days Abstract: Introduction: The goal of the ecological succession lab is to demonstrate succession with the fermentation of cabbage and cucumbers. The cabbage will ferment into sauerkraut and the cucumber will ferment to pickles during fermentation process that will changes the species structure and the community of time. During the fermentation process we watched the pH become more acidic‚ since
Premium Bacteria Acid Microbiology
Designation: D2972 – 08 Standard Test Methods for Arsenic in Water1 This standard is issued under the fixed designation D2972; the number immediately following the designation indicates the year of original adoption or‚ in the case of revision‚ the year of last revision. A number in parentheses indicates the year of last reapproval. A superscript epsilon (´) indicates an editorial change since the last revision or reapproval. 1. Scope 1.1 These test methods2 cover the photometric and atomic
Premium Analytical chemistry Reagent Hydrochloric acid
Jewellyn G. Diola; ladyjewellyndiola@yahoo.com Abstract Synthesis of 3‚4-dihydro-3-(p-methylphenyl)-1‚2-(2h)-benzoxazine involves the nucleophillic addition of the 1 °amine group upon the carbonyly group of the salicylaldehyde‚ the reduction of imine to amine and the addition of paraformaldehyde to proceed ring closure. The experiment prepared the product through Mannich reaction‚ a multicomponent condensation synthesis between ketone‚ aldehydes‚ enols and amines. Biosynthesis of benzoaxazines
Premium Amino acid
Paracetamol - a curriculum resource Frank Ellis RSeC ROYAL SOCIETY OF CHEMISTRY Paracetamol - a curriculum resource Compiled by Frank E llis Edited by Colin Osborne and Maria Pack Designed by lmogen Bertin Published by the Royal Society of Chemistry Printed by the Royal Society of Chemistry Copyright 0 Royal Society of Chemistry 2002 Registered charity No. 207890 Apart from any fair dealing for the purposes of research or private study‚ or criticism or review‚ as permitted under
Premium Sodium hydroxide Sulfuric acid Hydrochloric acid