Enzymatic Assay of TYROSINASE (EC 1.14.18.1) PRINCIPLE: L-Tyrosine + O2 L-DOPA Tyrosinase Tyrosinase > L-DOPA > L-DOPA-quinone + H20 Abbreviation used: L-DOPA = L-3‚4-Dihydroxyphenylalanine CONDITIONS: METHOD: REAGENTS: A. 50 mM Potassium Phosphate Buffer‚ pH 6.5 at 25° C (Prepare 50 ml in deionized water using Potassium Phosphate‚ Monobasic‚ Anhydrous‚ Sigma Prod. No. P5379. Adjust to pH 6.5 at 25° C with 1 M KOH.) 1 mM L-Tyrosine Solution (Prepare 100 ml in deionized water using L-Tyrosine
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Experiment #8: Limiting Reactant Abstract In chemical reactions‚ the significance of knowing the limiting reactant is high. In order to increase the percent yield of product‚ increasing the limiting reactant‚ possibly‚ is the most effective. In this experiment we were able to calculate limiting reactants from the reaction of CaCl2. 2H2O + K2C2O4.H2O(aq). As a group‚ we obtained our salt mixture of calcium chloride and potassium oxalate‚ and weighed the mixture. We were able to make an aqueous
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Percent By Mass Solution Problems 1. What is the percent by mass of a solution made by dissolving 0.49 g of potassium sulfate in 12.70 g of water? 2. What is the percentage concentration of 75.0 g of ethanol dissolved in 500.0 g of water? 3. A chemist dissolves 3.50 g of potassium iodate and 6.23 g of potassium hydroxide in 805.05 g of water. What is the percentage concentration of each solute in the solution? 4. A student wants to make a 5.00 % solution of rubidium chloride
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Noah Stewart-Maddox Chem 121 10/12/11 Synthesis of Potassium Ferric Oxalate Trihydrate and The Determination of Oxalate Ion in Ferric Oxalate Trihydrate using Titrimetry Abstract: In this two-part lab‚ we will learn about coordination compounds and their uses with stoiciometry. We will also find out about how theoretical yield is calculated from a reaction we will create. We will also synthesize Potassium Ferric Oxalate Trihydrate (K_3 [〖Fe(C_2 O_4)〗_3]•3H_2 O) using a two step reaction. In
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Safety Precautions for Synthesis of Acetic Anhydride Acetic Anhydride Acetic anhydride is an irritant and also flammable‚ therefore gloves and goggles should be worn at all times during the experiment. It is reactive to water‚ so in the case of fire‚ alcohol foam or carbon dioxide is preferred to use as an extinguisher. This chemical has harmful fumes and use of a fume hood is strongly recommended. Sulphuric and Salicylic Acid These acids may irritate the skin in high concentrations. Take
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1) Who should be immediately called for assistance in case of an accident in the laboratory? Your laboratory instructor. Your instructor should be trained to assist with emergency first aid until qualified medical assistance arrives. 2) What must be worn in the laboratory at all times to decrease the likelihood of an eye injury? Goggles or approved glasses. Contact lenses should be worn only with GOGGLES or FACE SHIELD. Anything getting under a contact lens will not wash out unless the lens
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Aug 10‚ 2011 - To the best of my knowledge‚ the Gr1 alkali metals cations ‚ ie Na+‚ K+‚ Li+ etc do not form precipitates. In addition the NH4 cation does not form a ... 3 questions on cations and anions? 2 answers Nov 23‚ 2011 AP Chemistry Problems? 1 answer Sep 9‚ 2011 What anions form precipitates? 2 answers Jun 25‚ 2010 What cations commonly form precipitates? 1 answer Oct 13‚ 2008 More results from answers.yahoo.com [DOC]Solubility Rules Lab 2 C12-1-02 umanitoba.ca/.../Solubility%20Rules%20Lab%20
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Unit 4: Basic Stoichiometry Rocky Mountain Poison and Drug Center Case Study DUE _________________________________ at Midnight All Sections are a level 3 Title and Date Is it present Identifies the independent and dependent variables. Describes the independent and dependent variables. Problem (level 3) Complete sentence (if a problem‚ in question form) Identifies the independent and dependent variables. Describes the independent and dependent variables. Case
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Andrea Moreno 4 September 2015 Block B Calculations: There are no calculations. Analysis/ Critical Thinking Questions: Compare your data for the pure substances with the data for each of the two solid mixtures. Which of the pure substances shows behavior that is similar to what you saw with the the two-solid mixtures (Mixture 1 and Mixture 2)? If you cannot positively identify your mixture‚ discuss the possibilities for the mixture. The starch and mixture 2 are both similar because they both did
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| Percent Yield Lab | | | | 4/20/2012 | Mrs.Sardella Per4 Matt ‚ Kait Mrs.Sardella Per4 Matt ‚ Kait | Introduction *Limiting Reactant: A reactant that is completely consumed during a chemical reaction‚ limiting the amount of product that is produced. *Excess Reactant: A reactant that remains after a reaction is over. *Theoretical Yield: The amount of product that is predicted by stoichiometric calculations *Actual Yield: The amount of product that is recovered
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