Title: Principles and Practice of Agarose Gel Electrophorsis Objectives: To detect the size ‚ shape and charge of the each dye solution. Methods: Casting the Agarose Gel In this experiment .8% solution was used. By using a 250ml flask the buffer solution was prepared. Using the equation to make enough solution for the intire lab class the equation had to be multiplyed by five. The contents of this equation were added to the 250ml flask and swirled to evenly distrubute it contents
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the gel after electrophoresis. Gel 1 Gel 2 Lane 7. This is Maddie’s (MCB) sample. Gel 2 Lane 6. This is Madi’s (MN) sample. From our sample of the gel electrophoresis‚ both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it‚ to find out if we are homozygous or heterozygous. Discussion For this lab‚ DNA from our cheek cells were separated through PCR‚ and singled out through gel electrophoresis
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How does the process of gel electrophoresis separate DNA fragments? It uses an electric current to separate different-sized molecules in a porous‚ sponge-like matrix. 2. What is the purpose of the agarose gel? It is used to separate DNA molecules that range in different lengths. 3. What is the purpose of adding blue “tracking” dye to the DNA samples? The blue tracking dye is added to help load the samples easily and helps able to see the DNA moving through the gel. 4. Explain why DNA has an
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CASE STUDY CASE OVERVIEW Company Background EEI Corporation was one of the oldest construction companies in the Philippines who is engaged in the business of building industrial plant facilities‚ installing equipments‚ providing replacements parts and supplies‚ and providing specialized engineering services to industrial companies in the Philippines and overseas‚ principally in Middle East. Highlights of Operations EEI Corporation struggled in mid 1980s where they faced financial difficulties
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Background Headquartered in Texas‚ Teletech Corporation operates under two main business segments: the Telecommunications Services segment‚ providing various telephone services to business and residential customers and the Products & Systems segment‚ which manufactures computing and telecommunications equipment. In late 2005‚ the Securities & Exchange Commission revealed that billionaire Victor Yossarian acquired a 10% stake in Teletech and demanded two seats on the board of directors. He felt
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4. DATA ANALYSIS & INTERPRETATIONS 4.1. Objective-1(A) determinants of EQ-Kensium Soft: - 4.1.1. Finding the correlation for employees in attention‚ clarity and Repair factors of EQ: - TABLE 4.1.1. Correlation of Attention factor in Kensium soft: - Item Correlation Item-1 0.2645396 Item-3 0.429901 Item-5 -0.030265 Item-7 0.3318278 Item-9 0.2327557 Item-11 0.4495084 Item-13 0.2866891 Item-15 0.1304 Item-17 0.1576763 Item-19 0.5485856 Item-21 0.2996818 Item-23 0.0978369 Item-25 0.4787094 Item-27
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------------------------------------------------- Introduction The 50-bn-rupee soft drink industry is growing now at 6 to 7% annually. In India‚ Coke and Pepsi have a combined market share of around 95% directly or through franchisees. Campa Cola has a 1% share‚ and the rest is divided among local players. Industry watchers say‚ fake products also account for a good share of the balance. There are about 110 soft drink producing units (60% being owned by Indian bottlers) in the country‚ employing
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Legalizing soft drugs such as Marianna can have its pluses and negatives. Both can be argued evenly with the same results. In this essay I will not choose sides‚ but I will explain the positives that Marijuana can offer as well as the negatives aspects of the drug. First I would like to explore some negative effects that marijuana can have on the human body. When marijuana is smoked‚ THC rapidly passes from the lungs into the bloodstream‚ which carries the chemical to the brain and other organs
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The short story‚ There Will Come Soft Rains by Ray Bradbury‚ was about a world without Humans. Nuclear war wiped people from the face of the earth and what remained‚ in a city‚ was just a house. A self sufficient and working house which was programmed to do certain things without the help of people. Even with the family gone‚ it remained doing the things it was programmed to do. With this short story‚ Bradbury tried to show that a world without reason and understanding is a Dystopian world The
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setting and plot of the story to the responder and thus fulfill its purpose. I will be illustrating this through the analysis of the Henry Lawson stories including “The Drover’s Wife” and “The Loaded Dog” and through other related texts “There Will Come Soft Rains” by Ray Bradbury and “Charles” by Shirley Jackson. These stories present a variety of techniques in order
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