EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine
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years questions with solutions Topic: Strength of Solution Q.1Discuss the effect of Temperature on Solubility of solids in Solvent. AnsThe solubility of solid in a liquid solvent generally increases with increase in temperature of the solvent. Very rarely some neutral salts like KCl for some solids the solubility decreases with increase in temperature. temperature increases‚ the solubility of a gas decrease as shown by the downward trend in the graph .More gas is present in a solution with
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possible substances. Theory Panacetin should be made up of about 50 percent of the unknown component that we previously separated out of Panacetin for testing. We suspect that this unknown compound is either acetanilide or phenacetin. From the solubility of acetaminophen and phenacetin‚ we could know both of them are relatively soluble in boiling water but insoluble in cold water so that we took the recrystallization as the method to purifying the unknown component. After the unknown purified‚ then
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was done using extraction‚ solubility‚ crystallization and vacuum filtration. The experiment was carried out two times‚ both of which were successful. Background Information: This experiment combined all the knowledge of the previous labs performed throughout the semester. An unknown mixture containing an organic acid or base and an organic neutral compound in nearly equal amounts needs to be separated to its separate components. An understanding of solubility‚ extraction‚ crystallization
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Honors 1-18-12 Relative Solubility of Transition Elements Introduction: In this lab we evaluate the solubility of compounds of iron‚ copper‚ zinc‚ and mercury in both water and acid. Coming into the lab we know that iron‚ copper‚ and zinc are all found in the the same row of the periodic table‚ while zinc and mercury are found in the same column. The transition metals are found in groups 3 to 12 and periods 4 to 6. Rows are known as periods and columns are known as groups. They share characteristics
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8.4 Water 8.4.1 Water is distributed on Earth as a solid‚ liquid and gas y y y Define the terms solute‚ solvent and solution Solution a homogeneous mixture of two or more substances Solute a substance that is dissolved in another substance or the component of a solution present in a lower amount. Solvent a substance which can dissolve another substance or the component of a solution present in a greater amount. Identify the importance of water as a solvent Water is essential as a reactant
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GRAVIMETRIC ANALYSIS Methods in GA Precipitation Method - involves isolation of an ion in solution by a precipitation reaction‚ filtering‚ washing the precipitate free of contaminants‚ conversion of the precipitate to a product of known composition‚ and finally weighing the precipitate and determining its mass by difference. From the mass and known composition of the precipitate‚ the amount of the original ion can be determined. Volatilization Method -the analyte or its decomposition products
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PURPOSE: To see which solutions are soluble and which are not. We were able to see this by mixing certain solutions together and observing changes that occurred. PROCEDURE: 1 Cotton Swabs‚ 1 Sheet each of white and black paper‚ 1 Distilled water‚ Goggles-Safety‚ 1 Well-Plate-24‚1 Well-Plate-96‚ Bag-CK1 1 Pipet‚ Empty Short Stem‚ Experiment Bag Ionic Reactions 1 Barium Nitrate‚ 0.1 M - 2 mL in Pipet‚ 1 Cobalt (II) Nitrate‚ 0.1 M - 2 mL in Pipet1 Copper (II) Nitrate‚ 0.1 M - 2 mL in Pipet‚ 1 Iron
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Gravimetric Analysis of SULFATE as Barium Sulfate. Objectives * To learn the techniques associated with gravimetric analysis. * To use stoichiometry to calculate the percentage by mass of sulfate in an unknown sulfate salt. Background Gravimetric analysis is a quantitative method for accurately determining the amount of a substance by selective precipitation of the substance from an aqueous solution. The precipitate is separated from the remaining aqueous solution by filtration and
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Punjabi February 16‚ 2017 RESULTS & DISCUSSION The purpose of this experiment was to utilize two different methods of qualitative analysis to determine the identity of two unknown cations in an unknown solution. The experiment required knowledge of solubility rules and demonstration of such in the laboratory setting for the first method of qualitative analysis. The second method of qualitative analysis was the performance of a flame test on various solutions to prove the presence of certain ions based
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