WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
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COST-VOLUME PROFIT (CVP) ANALYSIS This is a technique used for planning short-term run profits by finding the relationship between profits and factors that influence profits. The following factors are taken to be influencing profits:- • Selling price • Variable cost of production • Fixed costs • Activity level (production and sales units) Profit planning is based on break-even analysis and can be worked out using either; a) Algebraic method b) Contribution method c) Break-even
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Manual for Training employees of Aquavi Restaurant Jessica Gonzalez Food Service Sanitation American University Sheri Hernandez 26 August 2012 Table of contents Purpose and Scope of Manual 1. To ensure safe food …………………………………………………………….. 2 2. Prevent foodborne illnesses …………………………………………………….... 2 Foundations for Use 1. Food Safety Hazards …………………………………………………………….. 2-3 2. CDC the 5 main risks for foodborne illnesses ……………………………………. 3-4 Safe Food
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INTRODUCTION 1. ECOMMERCE In the simple language ecommerce is the buying and selling of goods and services online. People use ecommerce to describe a purchase when the payment is been made online. A sale on internet is increasing now a day as the customer takes the advantage of low prices by the wholesaler retailing the product. E commerce also include the entire process of developing ‚ marketing‚ selling‚ delivering‚ servicing and paying for product and service. Modern Ecommerce typically
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Western Sahara is a disputed territory between the Polisario Front and the Kingdom of Morocco. The territory in the North African‚ Maghreb region was formerly called Spanish Sahara when it was under Spanish colonial rule. Currently‚ the United Nations (UN) lists it as a non-decolonized territory and it is counted among the United Nations List of Non-Self-Governing Territories. (Fig 1 Map of Western Sahara showing the borders) Historical Background The dispute over Western Sahara can
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Slavery in the South A large proportion of whites in the South supported slavery even though less than a quarter of these whites actually owned slaves. They felt that slavery was a necessary evil and that it was an important southern institution. The slave population in 1800 was just under 900‚000 slaves and of that only 36‚000 of these slaves were in the northern states. In 1860 this number grew to almost 4 million slaves were in the southern states. Many important statesmen such as Thomas
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South Asia‚ which consists of the nations of Bangladesh‚ Bhutan‚ India‚ Maldives‚ Nepal‚ Pakistan‚ and Sri Lanka‚ is ethnically diverse‚ with more than 2‚000 ethnic entities with populations ranging from hundreds of millions to small tribal groups. South Asia has been invaded and settled by many ethnic groups over the centuries - including various Dravidian‚ Indo-Aryan and Iranian groups - and amalgamation of Dravidian‚ Indo-Aryan and native societies has produced composite cultures with many common
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COST-VOLUMEPROFIT ANALYSIS Julie E. Colandog A systematic examination of the relationship among cost‚ cost driver or level of activity (volume)‚ and Sales Less: Variable Costs Contribution Margin Less: Fixed Costs Net Profit xxxx xxxx xxxx xxxx xxxx CONTRIBUTION MARGIN INCOME STATEMENT e s Sa l Total Cost Break-even point Fixed Cost Break-even point is a condition where total revenue equals total cost and profit is equal to zero BREAK-EVEN POINT Break-even point (pesos) = Total Fixed
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Western Summit Constructors/Midterm Paper Western Summit Constructors‚ Inc. is an environmental contracting company that targets both industrial and municipal clients. The company was established in 1984 in the rocky mountain area and quickly grew to build projects all over the country. In 2008 Western Summit was purchased by Kiewit Corporation‚ one of the largest and most premier international contracting groups. The Western Summit division of Kiewit specializes in building water and waste water
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. South Africa Alicia Hudson SOC 315 Professor Norsworthy May 6‚ 2013 South Africa South Africa‚ a country on the southern tip of Africa‚ has an area of 471‚442sq mi and a population of 44‚188‚000. It is predominately a black ethnicity with 76% of the population. Although South Africa is Africa ’s most developed country‚ most of the black people - rural and urban - are poor‚ with low standards of living. South Africa has vital natural resources such as diamonds and gold and is rich in
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