solute particles is greater than the other solution. Core B was isotonic meaning that it had an equal balance throughout. When two environments are isotonic it means that the total molar concentration of dissolved solutes is the same in both of them (equal). When cells are in an isotonic solution‚ movement of water out of the cell is exactly balanced by movement of water into the cell. Core C was the most flexible which made it be hypotonic. A hypotonic solution expresses that the total concentration
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objective of this lab was to recreate the color profile of a given solution. In this case‚ the solution was Powerade. The final solution should match the absorbance values at the peak wavelengths (420nm and 628nm) in Powerade. This lab was done using deionized water‚ FD&C Blue #1‚ FD&C Yellow #5‚ FD&C Red #40‚ and a spectrometer. To obtain the correct color profile‚ FD&C Blue #1 and FD&C Yellow #5 were utilized in the sample solutions. The experiment was conducted over two days; the first day was reserved
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5.5 Candy Chromatography Background Information: Paper Chromatography is a separation tool in which pigment is put on a paper made of cellulose and water‚ and placed in a solvent‚ in this case isopropyl alcohol. Due to capillary action‚ the solvent crawls up the paper‚ separating the pigments. This technique is used to identify components of a mixture‚ even unknown ones‚ and can be used to isolate components into pure samples. Real world uses of this technique includes identifying certain biomolecules
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The first step is to calibrate the colorimeter with0.20 M Fe(NO3)3and set the absorbance at 470 nm since it is known to keep an acidic solution throughout the entirety of the experiment. It was important to do this right at the beginning of the lab since the zeroed value of the acid was the calibration number for all of the other solutions. A total of seven solutions with different dilutions were used throughout the lab to conduct the equilibrium constant. The first step was adding 5 mL of 0.200 M
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1. The standard curve is a graph that shows a relationship between concentration and absorbance of a solution. A standard curve was experimentally created in this experiment using 10mL solutions of phenol red with concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations
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concentration. The three types of concentrations are hypotonic‚ hypertonic‚ and isotonic. When in comparison to another solution‚ a hypertonic solution has a lower concentration‚ a hypertonic solution has a higher concentration‚ and an isotonic is when the two solutions have an equal concentration. The experiment tested the relationship between the concentration of an egg and solutions of different concentrations. The hypothesis is that an egg placed in distilled water will gain mass while an egg placed
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of maximum absorption‚ Amax of bromophenol blue. 2. To construct a standard concentration curve for bromophenol blue. 3. To determine the concentration of the unknown bromophenol blue solutions. 4. To determine the concentration of two different solutes‚ bromophenol blue and methyl orange‚ in a mixture. Material and method: Refer to practical manual page 7 Results: Part 1: Determination of Amax of bromophemol blue
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specific solutes to and from the cell. This implies that the membrane to most cells is selectively permeable or has a differential permeability to different solutes. Both the internal and the external environment to the cell are composed an aqueous solution that is made of dissolved organic and inorganic substances. The gradual or spontaneous movement of these substances in and out the cell are guided by a mechanism called diffusion. This is a movement by molecules to a region of lower concentration
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two different solutions. Osmosis has a lot to do with this experiment and is the movement of water molecules from a higher concentration to a lower concentration. Osmosis only deals with water and is a type of diffusion. The difference between all three solutions is that in a hypertonic solution the cells fluid rushes out of the cell and causes it to shrivel. In a hypotonic solution water rushes into the cell and causes the cell to expand and eventually pop. In a isotonic solution water rushes in
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however‚ deals solely with water. Osmotic pressure is the pressure of a solution against a semi-permeable membrane to prevent water from flowing into the membrane. In this lab‚ we are going to study tonicity; tonicity is the measure of this osmotic pressure and is the differential of pressure between two solutions separated by a selective membrane. To help identify the relative concentrations of solute particles of different solutions‚ we must understand that there are three possible differences in concentrations
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