During this experiment‚ the method of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was conducted using a negatively charged protein and pre-stained molecular weight markers. The hypothesis was that the molecular weight of N-acetyl-β-D-hexosaminidase B would be 28‚000 kDa. To confirm or reject the hypothesis‚ the molecular weight of N-acetyl-β-D-hexosaminidase B and the concentration of protein had to be determined. The electrophoresis of the protein gel were conducted using
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First‚ the synthesis of the coordination compound‚ KaFeb(ox)cdH2O‚ needed to be carried out. This was achieved by weighing out 8.29 grams of K2C2O4 with a Dial-a-gram balance. Once measured‚ the 8.29 grams K2C2O4 were placed in a 125 mL Erlenmeyer flask. 25 mL of deionized water were then added to the 125 mL flask and stirred with the K2C2O4 until complete dissolution was achieved. Next‚ 4.11 grams of FeCl3 were weighed out using the balance previously mentioned. Once the 4.11 grams were measured
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Introduction 3 Results 4 Discussion 6 Conclusion 7 Experimental 7 References 8 Preparation of 2-Acetylcyclohexanone Introduction When hydrogens are present on the α-carbon of ketones (like the reaction used in this experiment)‚ carbonyl compounds and aldehydes‚ they turn the compound slightly acidic1. These functional groups are removed by using a basic solution as shown in (i) below1. The product formed with water is stable only due to resonance‚ but does
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Chemistry Project on Evaporation of Liquid Acknowledgement I would like to show my gratitude to my Chemistry Teachers Ms Seema More without whose support and encouragement this project could never have have taken the present shape . I would also like to thank my lab incharge Mr. Kakde For helping Me out During The Practical Part Of This Project. Aim Acknowledgment I am are greatly thankful for the cooperation and help from the Community Science Center (CSC) authorities and the Chemistry lab
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STERILIZATION OF WATER USING BLEACHING POWDER A CHEMISTRY INVESTIGATORY PROGECT ANSHUL KUMAR PANDEY XII B [THIS PROJECT LOOKS AT THE TECHNIQUE CALLED STERILIZATION OF WATER USING BLEACHING POWDER WHICH IS USED TO PURIFY WATER AND MAKES IT FIT FOR DRINKING.] Downloaded from www.icbse.com INDEX 1. Certificate of Authenticity 2. Acknowledgement 3. Introduction -Need of Water -Purification of Water -Need for a stable purification technique 4. Theory -History of Water purification -Bleaching
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The Grignard Synthesis of 3-methyl-3-heptanol In this experiment‚ an example of an organometallic compound which has a carbon magnesium bond will be utilized to form a tertiary alcohol. Grignard reagents have been extremely useful in the synthesis of a large number of classes of organic functional groups. Although Grignard reagents are unstable and decompose in air and moisture‚ they can be prepared and used immediately with moderate difficulty in the undergraduate organic chemistry laboratory
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Abstract Pyrorle-2-aldehyde and 1‚3-diaminopropane react under reflux to form a transition metal ion of Nickel(II)‚ that further more react with nickel acetate to form red crystals through condensation‚ the colour being constituded by the congugated bonds. These series of reactions synthesize the Schiff Base ligand and Nickel(II) complex of the Schiff base ligand. 0.36g of the Schiff base ligand is yielded and 0.1g of the nickel complex is yielded from the 0.36g Schiff base ligand and 0.5g nickel
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OSMOSIS LAB BACKGROUND INFORMATION. Dialysis tubing is being used to model a plasma membrane. Isotonic solutions are solutions that have the same concentration of solutes on both sides of the membrane. Hypertonic solutions are solutions that have more solute to the one with less solute. Hypotonic solution are solutions that have less solute to the one with more solute. RESEARCH QUESTION:which dialysis tubing will gain the distilled water and which one will loss its content
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HPLC detection and quantification of polyphenolic compounds Detection and quantification of selected phenolic compounds in the ethanol extract were determined by HPLC-DAD analysis as described by Ismet et al. [13] with some modifications. It was carried out on a Dionex UltiMate 3000 system equipped with quaternary rapid separation pump (LPG-3400RS) and photodiode array detector (DAD-3000RS). Separation was performed using Acclaim® C18 (5µm) Dionex column (4.6 x 250 mm) at 30 ºC with a flow rate of
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There are several methods to test syneresis. Acid curds need to be formed in a glass beaker which make it easier to transfer the curds into syneresis test installations. All curds must be transferred carefully into the device‚ otherwise some soft curds were easily broken and the results would be affected. In case that some of the gel were not easily removed with the sample residues on the bottom of the beaker when transforming‚ beakers can be pre-coated with paraffin wax on the inside. Both a barrel-shaped
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