high demanding textile material. Plantation owners couldn’t work the whole plantations by themselves. The southern states depended heavily on slaves to work their plantations. The south depended heavily on slavery‚ and slavery was vital to the south because they needed the slave’s labor to work their plantations. This why slavery was so important to the southern political leaders. If the southern states didn’t have slavery their plantations would have most likely failed and they couldn’t produce anymore
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required bringing in food raised elsewhere. But‚ to continue the King Cotton economy on that scale in the absence of mechanization‚ slaves had to be continually purchased when “natural increase” could not supply labor demand. 2. Slavery encouraged the Southern economy‚ especially in the Deep South‚ to specialize in intensive agriculture‚ cotton in particular. It was a labor intensive crop and could only be profitable if pursued on large scales with vast armies of workers. Investment in this one crop and
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Southern Slavery versus Wage Slavery There are various accounts in the world in which the setting or time period plays an infinite roll‚ but in Harriet Jacobs‚ “Incidents in the Life of a Slave Girl”‚ and Rebecca Davis’s “Life in the Iron Mills”‚ the characters make all the difference. From the amazing role of Hugh Wolfe‚ to the vital words from Harriet Jacobs‚ we will explore how these stories have shaped our past‚ present‚ and future. Most people have experienced challenges in life that cause
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from the southern perspective. Although many northerners and southerners were ambivalent toward splitting their organizations and‚ as a result their resources‚ division was nonetheless the eventual result. The two sections could not reconcile their conflicting priorities‚ so the only logical answer to them‚ even in light of their shared religious beliefs‚ was to go their separate ways. This separation would have long-lasting repercussions in Baptist life. Even to the present the Southern Baptist Convention
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INTRODUCTION A major obstacle in the successful treatment of cancer is the development of resistance mechanisms to drug treatment. Various cellular changes that have been implicated in the development of drug resistance in cancer cells include: the increased expressions of P-glycoprotein (a multidrug transport protein) and multidrug resistance-associated protein (MRP-1); increased levels of the cellular detoxification protein‚ glutathione; and changes in the expression of apoptosis associated
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(Declaration of Independence 1776) The southern States now stand almost exactly in the same position as our ancestors did some 85 years ago. The difference is that Great Britain made no pretense that we were equal and had an equal say in matters. The Northern States‚ having the majority in Congress‚ claim the same power over us in legislation as the British Parliament did. The government of the United States has become a consolidated Government‚ and the people of the Southern States are compelled to meet
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an infectious food borne disease called Human Trinchinellosis. Human Trinchinellosis is a disease caused by ingestion of infected pork‚ meat and other foods in which animals can be exposed to this parasite. He analyzed sera from pigs using a western blot‚ for the detection of anti-Trichinella-IgG. The results from his experiment using western blotting was that 144 of the sera from pigs were Trichinella-free and 159 pig sera were infected. His study and our experiments are similar‚ but instead of
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antibodies‚ anti-immunoglobulin antibodies coupled to an enzyme alkaline phosphatase or horseradish peroxidase are added. This anti-IgG enzyme is commonly called a "second antibody" or "conjugate". Finally‚ after excess second antibody is washed free of the blot‚ a substrate is added which will precipitate upon reaction with the conjugate resulting in a visible band where the primary antibody is bound to the protein. The procedure can be broken down into a series of steps: 1. SDS Polyacrylamide Gel Electrophoresis
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2.4. Animals One hundred twelve adult male Wistar rats (Pasteur Institute‚ Tehran‚ Iran) weighing 220-250 g were housed in groups of four per cage in a controlled environment (temperature 23±1°C‚ 12h light/dark cycle) with free access to food. Rats were habituated to the new environment for seven days before the test. All experiments were performed in accordance with the guide for the care and use of laboratory animals (IR.SBMU.nrc.REC.1390.20) approved by the Research and Ethics Committee of the
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Review Sheet Exercise 12 Serological Testing NAME Kali Rothfuss LAB DATE/TIME 6/22/11 Positive and Negative Controls 1. Why are there a number of washing steps in serological tests? The are a number of steps needed in order to remove any non specific binding that may have occurred. 2. Describe how you would know that you had a “false positive” result. What does this mean for the rest of your results? A positive result with a negative control indicates a “false positive”
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