The samples were boiled for 3 minutes and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed
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Slavery was the dominating reality of all Southern life. The effects of slavery in the south can clearly be seen politically and economically. Cotton was the cash crop of the south and the government was white. This means that slavery was not going to disappear without a major event in the South that changed everything. In America during that time‚ a slave was a Piece of property just as our laptops‚ cars and houses are. This means that they can be bought‚ traded and loaned just as all of those
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Pacific Medical Supply Company Hiring‚ Motivating‚ and Retaining Good Salespeople Jim Shine had some big problems on his hands as the owner and sales manager of Pacific Medical Supply Company. Jim’s problems involved his most junior salesperson‚ Nicole Landis. Nicole’s impact on Pacific Medical Supply’s sales was huge‚ despite the fact that the company hired her right out of college. The specific problems Jim faced as a result of Nicole’s extraordinary sales efforts involved professional jealousy
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Clark Farley AMST 486: Shalom Y’all Dr. Marcie Cohen Ferris 08 December‚ 2010 The Relationship of Southern Jews to Blacks and the Civil Rights Movement Since the 1960’s historians and many other scholars have tried to delve into the relationship of blacks and Jews. The experiences of blacks and Jewish people have common histories of dispersion‚ bondage‚ persecution‚ and emancipation. Their relationship can be primarily recognized since the formation of the NAACP in 1909. During the
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1. What are antibodies and why are antibodies ideal for targeting? An antibody‚ also known as an immunoglobulin‚ is a large Y-shaped protein used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target‚ termed an antigen.[1][2] Each tip of the "Y" of an antibody contains a paratope (a structure analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen
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events that occurred in the United States of America. The Civil War‚ also appropriately known as the War between the States‚ was fought from 1861-1865. This war involved the rivalry between the states of the Northern and Southern territories. The War was declared when the Southern states declared their secession and formed the Confederacy‚ a new government‚ of the south. There has been many disputes over which societal events led up to this war and many scholars have tried to narrow down which might
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In order to able to detect and analyze proteins based on their ability to bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common technique used to separate proteins based on molecular weight under the influence of an applied electrical field and then used to prepare for the Western Blot (#1 Lehninger). The support medium used is a polyacrylamide gel and‚ it also used sodium
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Stephany Martin Period 1 1.12.14 Northern and Southern views on liberty during the Civil War There are many different ways to define liberty and many different ways to define who is entitled to liberty. Many people can disagree on specific issues and yet still claim to both be supporting liberty. During the time leading up to the Civil War and the Civil War itself‚ northern whites who opposed slavery and southern whites who supported slavery both believed they were fighting for liberty.
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the Union was a result of the schism separating northern and southern states‚ originating from differing opinions on slavery which ultimately incited the South’s secession. As the election of 1860 drew near‚ hopeful democratic and republican candidates emerged despite the already turbulent condition of the nation over the issue of slavery. The Democratic ballot was split between the northern representative Stephen Douglas and southern representative John Breckinridge‚ while alone on the Republican
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Lincoln terrified the southern states. Southerners new that Lincoln favored abolition and he desired to rule against slavery in the western territories. There was a fear that Lincoln would eventually outlaw slavery for good. Therefore‚ the southern way of life would forever be changed. The southern states came together and decided the best solution would be to secede from the union. South Carolina was the first state to request to leave the union. Consequently‚ more southern states followed their
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