3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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Chameleons‚ translated from the Portuguese by Daniel Hahn. Set in contemporary Angola‚ the book is particularly notable for being narrated by a lizard. The judges this year were the poet David Constantine‚ writer and editor Jennie Erdal‚ Arts Council Literature officer Kate Griffin‚ novelist Ali Smith and the literary editor of the Independent‚ Boyd Tonkin. Admirably the prize is shared between the translator and author‚ thus honouring an art that often goes unsung. The book was evidently a popular choice
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Literature and Life Debora C. English 125 Professor Daw March 28‚ 2011 Literature and Life The magic of reading is that it brings you to another place and feeling. At times it can make you smile with character‚ and other times make you cry with him. Sometimes it may even allow the reader to identify with the characters. Reality can often be a lot like a piece of literature‚ in that a person may be going through the exact same thing‚ or something similar‚ and be feeling the same way. It
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piece of work. Memory‚ symbol‚ and patterns help us understand the language of reading. These three things must be applied when reading a piece of literature. Symbolic imagination is the ability to look beyond the plot of the story and see the patterns inside the book. This is an important skill to have when trying to understand the language of literature. 2) Foster means that all stories take ideas from other stories. There is no story that is completely original without any similarity to another
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The southern economy flourished on the backbones of the men and women that were enslaved in the 18th and 19th centuries. With the threat of the removal of the core method of profit for hundreds of plantation owners‚ an anger rose that would lead to an illogical reaction of withdrawing their entire region from the country that had built them. In essence the South felt a strong need to protect what they believed they possessed: the use of humans as components in the profit machine. This fierce protectiveness
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A Good Man Is Hard To Find Flannery O’Connor was one of the most known authors for writing southern gothic short stories. Southern gothic became a style of writing in the wake of the civil war and brought up questions in society like‚ ‘Why is violence such a large part of the south’s culture?’ and‚ ‘How did the South have such a hard time picking itself up after its defeat in the war?’ Southern gothic is usually decayed‚ grotesque‚ or derelict settings and situations and had themes of ambivalent
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Southern Colonies The southern colonies consisted of five of the first thirteen colonies‚ which were North Carolina‚ South Carolina‚ Georgia‚ Maryland‚ and Virginia. Where the climate was the warmest out of the three colonial regions‚ which to those living there was a good thing because survival in the winter was easier for them. Even back in the day‚ money has been an issue for many. This is why many colonist from New England decided to move to the Southern Colonies. In the southern colonies‚
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1. State the meaning of literature; 2. Discuss the importance of literature; 3. Appreciate the extent of literature in all aspects of human environment; 4. Familiarize yourselves with the types of literature; Compare and contrast the four literary genres 1.1 Topic - What is Literature? Literature seems too difficult to embrace to people who are indifferent to it. However‚ it continues to grow and stay because of its significance and value to man. Literature is an art of self-expression
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There are few works that truly stand alone when it comes to literature. As such‚ many allusions to other works are brought up whether explicitly or unbeknownst to even to author An allusion is an expression designed to call something to mind without mentioning it explicitly; an indirect or passing reference. However‚ in the poems “On first looking into Chapman’s Homer” by John Keats and “When I Consider How My Light is Spent” by John Milton‚ the authors explicitly allude to specific works to incite
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Drama Song of Roland Boethius=Consolation of Philosophy Commedia del Arte Roman de la Rose Dante=Divine Comedy La Vita Nuova Boccacio=Decameron Petrarch ENGLISH LITERATURE ANGLO –
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