Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
Premium Plasmid Escherichia coli Agar plate
Crystallization: Lab Report Theory/Introduction The purpose of this lab is to understand the concept of purification through crystallization. Crystallization is the process of purifying a substance‚ with the most adequate solvent. The process of crystallization came to be based on the principle of solubility. First‚ we must choose a solvent. The solvent is crucial to crystallization because it’s what will allow impurities to dissolve. Therefore‚ when selecting a solvent it is important that it
Premium Solubility Chemistry Solution
Feb 2015 Lab group Mech 7 Introduction In this lab report we show the basic methods of measuring centrifugal force using two counter balanced bell-cranks spin on a turntable (shows in figure 1) and able to calculate the centrifugal force with given conditions shows in figure 1. *Figure 1 (University of Birmingham. Experiments and Statistics‚ 2014.) Objectives 1. To calculate bending moment at the “cut” using displayed force (figure 1) times the perpendicular distance
Premium Force Torque Experiment
CO2 Lab Report Intro Alka-Seltzer tablets are used to treat cold/flu symptoms as it produces CO2 when dissolved in water. The goal of this lab is to calculate the amount of NaHCO3 is needed to produce enough CO2 gas to fill the room. Experimental One Alka-Seltzer tablet was weighed on the analytical balance. Then a piece was measured out to be between 0.21 and 0.28 grams. 500mL of distilled water was added to a 600mL beaker. 80mL of water was mixed with 20mL of 6M HCl in a 150mL beaker. A
Premium Tablet Laboratory glassware
BIOLOGY LAB REPORT (UNIT 7: ENZYMES) GENERAL Enzymes are protein that acts as catalyst‚ lowering the activation energy need for reactions to progress in cells. The reaction can still occur without the presence of the enzyme‚ but at a much slower rate. The activation energy is the minimum amount of energy need for a chemical reaction to occur‚ yielding from a given set of reactants. In enzymatic reactions‚ we have substrates which are reactants of reaction bound to an enzyme. While an active site
Premium Enzyme Catalysis
Formal Lab Report #1 I. Basics Title of the Experiment: The Empirical Formula of an Oxide Authors: Section Number: Chemistry Location and Date II. Abstract The aim in this lab was to determine the empirical formula of an oxide of magnesium through combustion in air. This was achieved by heating an established mass of magnesium in air inside of a crucible‚ ultimately attaining a compound that contained Mg and O. The major result of the experiment was the empirical formula of the oxide of magnesium
Free Oxide Oxygen Chemistry
HAEF Psychico College ENVIRONMENTAL SYSTEMS & SOCIETIES LAB REPORT (SL) TITLE OF LAB : Investigation of the impact of overpopulation in biodiversity Objective of the investigation: To investigate the impact of climate change in biodiversity Research Question: How does the temperature change ( each pot will be held in 35°C‚ 20°C‚ 10°C‚ 0°C‚ -10°C respectively) influence the growth of lettuce plants calculated from the measurement of the weight of each plant every 2 days
Premium Plant Water Light
Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
Premium Gram staining Staining Microbiology
General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
Premium DNA Molecular biology Electric charge
Abstract: Bacteria consists of a large domain of prokaryotic microorganism in which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly
Premium Bacteria Antibiotic resistance