The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Introduction In this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps
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salt solution is 6.3grams and the 15% salt solution is 6.8 grams. My hypothesis was that the carrot would shrink and be very small and gooey‚ but it was wrong. The controlled variable in this lab is the carrot and time‚ the manipulated variable is the salt solution‚ and the resulting variable in this lab is what happens to the carrot. Materials and Methods: . Salt solution ( 0-15%) . 4 Carrot pieces from same Carrot( Cut carrot in half if needed) . 4 Beakers . Water . Triple beam balance
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Mr. Mihordea Motion of Atoms and Molecules lab summary In this lab the standard heat of combustion of magnesium was calculated by using the calorimeter. Also‚ the point of combustion of magnesium was to see how much heat would be released from fireworks such as sparklers. The concept of this lab was to find the difference in temperature from the beginning to end of combustion of magnesium and calculate the standard heat
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Ketchup Lab Report Problem: People visiting fast food restaurants complained that ketchup was dripping onto their fingers and clothes. What could we do to insure that the ketchup will not drop all over the customers? Hypothesis: If I put one ketchup packet in a hotter bath then it will move faster down the styrofoam plate. Independent Variable: The temperature Dependent Variable: The viscosity of the ketchup’s “runniness” Constants: The type of ketchup
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Maria Martinez Anatomy and physiology II TTH 2:20 “Demonstrating the Importance of Surfactant: Lab Report” Introduction Surfactant is an essential component for the respiratory system to function properly. This experiment was administrated to help us understand better the role of surfactant in the alveolar system. Surfactant is a detergent-like substance produced by the Type II alveolar cells in the alveoli. Surfactant is produced to make it easier for people to breath by reducing the surface
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Biophysical Ecology Abstract: There are many patterns that can be observed throughout our environment. In this experiment‚ the temperatures of organisms in a biophysical environment were analyzed to see if a pattern could be recognized that was related to the behavior of an ectotherm. An ectotherm is an organism that relies on the environment to regulate its body temperature. Organisms such as Pseudemys (turtles) and Lacertilia (lizards) are examples or ectotherms. After arriving at Maxcy Gregg
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The purpose of the lab was to measure the effectiveness of antacids at neutralizing excess stomach acid. A neutralization reaction is when an acid and a base react to form water and salt. To illustrate‚ H+ hydrogen ions from the acid react with the hydroxide ions OH- from the base. Antacids are taken to relieve heartburn or indigestion caused by excess stomach acid. For instance‚ antacids such as alka-seltzers contain baking soda which is highly reactive with acids‚ settling the discomfort of indigestion
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purpose of antimicrobial drugs is either to inhibit virus replication‚ without harming host tissues or to interrupt the cell processes or structures of bacteria‚ fungi‚ and protozoa. This lab has brought great interest to me because this is the way physicians’ control/treat infectious disease within our bodies. In this lab‚ chemotherapeutic agents were evaluated by the disk-diffusion method. Chemotherapeutic agents are placed on the surface of a Petri plate containing Mueller-Hinton agar‚ which allows
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Exocytosis Lab Report Rough Draft Abstract: In Paramecium caudatum‚ trichocysts are 10^3 secretory vesicles that are released from the cytoplasm. We first observed how the presence of Alcian blue dye triggers normal secretion of trichocysts‚ and we then tested four different hypotheses in order to determine how the presence of Ca++ ions in the extracellular and intracellular environment affects the secretion of trichocysts. Our first our hypothesis tested whether extracellular calcium is
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