Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Daphny Maldonado Bio Lab 2107 Kiah Britton W 10-12:30 Is H20 Bad for You? Abstract: In the village of Gopher Hollow there’s a cluster of Blue Baby Syndrome. There were four infants affected by this cluster. The families from the infants would collect their water from wells. We have to determine what’s the source of the high levels of nitrites in the water. The four sources that could be the point of contamination are a new subdivision‚ textile plant‚ an organic farm‚ and a mountain lake
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Lab Report 7: Analysis of Cereal Introduction: The objective of this lab was to consult for the FDA regarding a recently surfaced scandal involving false reporting of iron content in cereal as well as iron tablets. The makers of the cereal and the iron tablets‚ respectively‚ were allegedly reporting higher amounts of iron in their products than actually existed‚ as a way to save money but continue to provide products with “adequate” amounts of iron. The FDA needed consulting in order to analyze
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Biochemistry is the chemistry of biological systems. The practical component of biochemistry is aimed at developing your interest in and understanding modern biochemical and molecular biological experimentation. The techniques learnt in the biochemistry lab will be applicable to all life sciences. THE OBJECTIVES OF THE BIOCHEMISTRY LABORATORY INCLUDE: (1) Learning the theory behind the techniques and biochemical pathways (2) Learning the physical skills and techniques of modern experimental biochemistry
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of the specialized underlying structures of these life-forms. In order for us to appreciate these special adaptation‚ we first need to know how a typical plant or an animal cell organelle behaves in different water and solute concentrations. In this lab‚ we will determine the effects of hypertonic‚ isotonic and hypotonic solutions on plant and animal cells. In general when an animals cell’s placed in hypertonic solution it shrivels; a plant cell on the other hand undergoes plasmolysis. When an animal
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The overall experiment consisted of three smaller experiments as a result of the different properties chlorophylls possess and the need to test them individually to receive the most accurate results. Preparation for the lab began with selecting a medium to large spinach leaf and deveining it. Once the spinach was prepared‚ it was ground in 5 mL of 100% acetone‚ creating a green acetone extract. The extract was filtered through a funnel with a kimwipe and into a test tube. The volume of the filtered
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Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
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Lab 5 The Diffraction Grating Chinua McDonald Objective: To measure the wavelength of light with a diffraction grating. Theory: The two types of diffraction gratings are the transmission and reflection gratings. They are made by ruling on a piece of glass or metal a number of evenly spaced lines with a fine diamond point. Diffraction phenomena can be analyzed in terms of Huygens’ principle‚ according to which every point on the wave front of a wave should be considered as a source
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indicated by a very pale pink color. To calculate the molarity of NaOH‚ the following equation was used MNaOH x VNaOH = MKHP x VKHP therefore the molarity was .125 M. INTRODUCTION This lab experiment covers the preparation of standard solution and the acid/base titration. The first part of the lab is to prepare a standard solution of Potassium hydrogen per. A standard solution is a solution of known concentration‚ in which it is prepared using exacting techniques to make sure that the molarity
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Materials ● ● ● ● ● ● ● Substrate solution Cuvette Spectrophotometer Timer Enzyme solutions Pipette for transferring fluids Thermometer Methods ● There is a choice of six different enzyme & substrate combinations in which the temperature will vary at which the reaction as well as the enzyme & substrate concentrations. pH: 1. A SUbstrate B/ Enzyme B was selected along with setting the wavelength to substrate vial/ 680 & the spectrophotometer to read light absorption. 2. The temperature was set to 25 degrees & set to different
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