concentrations can be used by diluting the concentration. The dilution technique is: Number Moles Concentrated Solution = Number Moles Dilute Solution. An instrument called a spectrophotometer detects the amount of light that passes through the sample and the percent transmittance can be recorded from the meter. In the lab‚ multiple homogeneous solutions are made. There was not a way to determine the differences in concentrations‚ but the Spec 20 made it possible to measure the difference. The Beer-Lambert
Premium Chemistry Concentration Solution
The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
Premium Protein Spectroscopy Electromagnetic radiation
Moment of Inertia and Rotational Motion Garret Hebert PHY 2311 Tues 1:00 garret.hebert@hindscc.edu Abstract: During this lab we will study what rotational Inertia is and how different shapes of masses and different masses behave inertially when compared to each other. We will specifically study the differences of inertia between a disk and a ring. We will use increasing forces to induce angular acceleration of both a disk and a ring of a certain mass. We will then then measure the differences
Premium Classical mechanics Inertia Torque
Through Chloroplasts Introduction: 1)Background= 2) Purpose= measure the rate of photosynthesis in chloroplasts. 3) The chloroplast will be subjected to two experimental conditions- light‚ and the absence of light‚ using a spectrophotometer to determine the amount of DPIP reduced at specific time intervals under each condition. 4) I predict the amount of DPIP reduced will vary for each condition and increase over the time intervals. I hypothesize under the light condition the
Premium Photosynthesis Light
analysis was important in an everyday context because ... Safety Information: During this lab nitric acid will be used as an oxidizing agent and (NO2) nitrogen dioxide will form. This gas is extremely toxic‚ and should not be inhaled. A use of a fume hood should be used during the process of adding nitric acid (HNO3) to the solution. Additionally‚ safety goggles should be worn throughout the entire lab. Shoes should be closed toed‚ clothing should cover
Premium Chemistry Chemical reaction Copper
Catecholase (an enzyme) to catechol (substrate) will result in the creation of Benzoquinone. Materials & Methods Adjusted the spectrophotometer with blank solution‚ then measured 1ml of Catecholase (potato extract) in the test tube B‚ using 1 ml graduate pipette and added 5 ml of distilled water. Protected the test tube with Parafilm and mixed the content. On the spectrophotometer‚ arranged the wavelength to 540 nm‚ formerly inserted the test tube into sample
Premium Enzyme Catalysis
Back Laboratory Program SAFETY IN THE CHEMISTRY LABORATORY 786 Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic
Premium Water Chemistry Drinking water
cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
Premium Bacteria
Solution ‘Concentration’ Using A Spectrophotometer” lab was to use a spectrophotometer to find the relationship of concentration and absorbance obeying the Beer-Lambert law‚ which states concentration and absorbance are directly related‚ to then further determine the concentration of three unknown solutions. With the assumption that the solutions obey the Beer-Lambert law it is predicted that as concentration increases‚ absorbance will increase as well. A spectrophotometer is a device that uses a light
Premium Chemistry Spectroscopy Light
Introduction Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is: Where [A] and [B] are the concentration of the species in the reaction. The variable k is the rate constant‚ which is a function of time and catalyst presence. The variables m and n are the order of reaction for their respective species concentration. The higher the value of the reaction order the
Premium Rate equation Chemical kinetics Reaction rate