Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a &
Free Electromagnetic radiation Light Spectroscopy
For this lab it was necessary to bring a watch with a second hand‚ as well as personal protective gear: lab coat‚ safety goggles‚ and safety gloves. It was best to work in pairs and one partner needed to be a timekeeper while the other one would record the data. The timekeeper then would announce every 5 second interval‚ beginning from when the enzyme was added to the tube. On the other hand‚ the recorder would read and record the absorbance from the spectrometer at the 5 second intervals. This was
Premium Chemistry Enzyme Water
always 1 cm for spectrophotometers.
Premium Chemistry Concentration Temperature
cells of all living organisms such as hormones‚ enzymes‚ antibodies. [1] Fig. 1 Fig. 2 General structure of amino acids Protein fragment showing peptide bond Basis of Spectrophotometer Measuring amount of substance in solution by helping of solution color is called colorimetry. Devices that used for this method is called colorimeter. In colorimetric measurements‚ color of solution is compared with color of standard solutions
Premium Amino acid Protein Concentration
The purpose of this lab was to use a spectrophotometer to find the concentrations of glucose in varying samples of Gatorade. The varying executions included changing the temperature of reaction (room temperature instead of 40 degrees centigrade)‚ changing the time for reaction (20 minutes in the water instead of 45 minutes)‚ and changing the amount of enzyme added (0.1mL of enzyme. The results in from this lab gave data points with respect to ferricyanide. Fortunately‚ ferricyancide and glucose have
Premium Chemistry Carbon dioxide Ethanol
Objectives: 1. To determine the wavelength of maximum absorption‚ Amax‚ of bromophenol blue. 2. To construct a standard concentration curve of bromophenol blue. 3. To determine the concentration of the unknown bromophenol blue solutions. 4. To determine the concentration of two different solutes‚ bromophenol blue and methyl orange in a mixture. Materials and Methods: Refer to Biological Science practical manual page 5-11. Results: Part 1: Determination of Amax of bromophenol blue The wavelength
Premium Linear regression Solution Concentration
Technical Specification Sheet For UV-Spectrophotometer | | Wavelength range | 190 to 1‚100 nm | Spectral bandwidth | 1 nm (190 to 1‚100 nm)/ variable Low stray light < 0.12%T (220 nm‚ NaI 340nm‚ NaNO2>2.0 Abs) | Wavelength accuracy | ± 0.1 nm at D2 peak 656.1 nm‚ | | ± 0.3 nm for entire range | Reproducibility | ±0.1 nm | Resolution | 1 nm more than 6 wave lengths | Photometric system | Double beam optics | Photometric range | Absorbance: -4 to 4 Abs | | Transmittance:
Premium Hertz Wavelength
Group No. 10 Date Started: June 11‚ 2013 Tongco‚ Val Justin DM. Date Finished: June 18‚ 2013 Santos‚ Erickson S. Vasquez‚ Jona Candace P. Wong‚ Victor Lorenzo E. Experiment 1 Use of Micropipettor and Spectrophotometer Introduction In scientific experiments‚ correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the
Premium Accuracy and precision Measurement
: Joanne Wong Student ID : 00000012636 (BM1/14) Title : Spectrophotometer and its function Introduction Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter‚ UV spectrometer‚ IR spectrometer‚ atomic spectrometer
Free Concentration Solution Light
This lab report will be detailing the steps taken and the results discovered when using spectrophotometry to determine the percentage of copper in a copper-clad penny and the thicknes of the copper layer on the copper-clad penny. After 1982‚ copper coating has been used in the creation of the penny because the cost of pure copper has increase to the point that the amount needed t omake a penny cost far more than the actual value of the penny. This lab allowed us to see just how much copper coating
Premium Chemistry Spectroscopy Ammonia