In this report‚ the concentration of total protein and albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol
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Report: Introduction: The experiment was conducted to find the unknown molarity of HCL. The unknown molarity of HCL was found by the use of the titration method and then comparing the results between the titrant and analyte HCL solutions. Molarity is a concentration unit‚ it is defined by the number of moles the total volume of the solution. This experiment included a process called titration‚ used to work out the unknown concentration of a known substance. The process of titration is adding titrant(known
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Title: Experiment 17‚ Reaction kinetics – Determination of the activation energy of the reaction between oxalic acid and potassium permanganate. Objective: To determine the activation energy of the reaction between oxalic acid and potassium permanganate. Introduction: Chemical kinetics is the study of chemical reaction rates‚ how reaction rates are controlled and the pathway or mechanism by which a reaction proceeds from its reactants to its products. Reaction rates vary from the very fast‚ which
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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used (ml) | 30.6 | 30.2 | 30.0 | | | | | Acid to Base Ratio | 0.87 | 0.86 | 0.86 | Average acid/base ratio | 0.86 | | | | | Base to Acid Ratio | 1.14 | 1.16 | 1.17 | Average base/acid Ratio | 1.16 | Name: Jared Philip Condez Date Performed: June 28‚ July 2 & 5‚ 2013 Partner: Shiela Mae Molina Date Submitted: July 12‚ 2013 Experiment 3 ACID – BASE TITRATION I. Objectives * Determine the purity of Potassium Acid Phthalate * To titrate effectively
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Experiment 2: Estimation of protein concentration Introduction Protein assays are designed to measure the total protein in a solution. Protein assays are quantitative if the protein to be assayed is available in sufficient quantity such that one is able to use it to create a standard curve. If this cannot be achieved‚ then a standard protein‚ such as albumin‚ may be used for a standard curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins
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Experiment #2 “Density Determinations” Report The objective for experiment #2 was “to determine densities of objects/salt solutions with different concentrations of salt‚ to see how density changes as a function of concentration.” In experiment #2‚ part II‚ calculations of Density of NaCL solutions were made from 0%-25% NaCL concentration. My hypothesis was that as the % increased‚ so would the density‚ because adding weight would increase the density of each solution. The density calculations
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Title: ACID BASE TITRATION. Objectives: 1. To determine the concentration of acid using titration. 2. Skills of titration techniques. Apparatus: 1. 250 volumetric flask 2. 10mL measuring cylinder 3. 25mL pipette 4. 50mL burette 5. 250mL beaker 6. 150mL conical flask 7. Retord stand 8. White tile 9. Stopwatch 10. Pipette bulb Chemicals: 1. HCl solution 2. 0.1M NaOH solution 3. H2SO4 solution 4. Distilled water 5. phenolphthalein Introduction
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report required the successful identification of two unknown substances through experimental testing on a selective collection of materials and equipment. For the first substance (which will be either tin or aluminium)‚ two tests shall be done to ensure a decisive and educated decision onto what the substance is. The first step utilizes the dramatic difference in density between the two substances by measuring the volume and mass of the unknown substance. By calculating the volume (through displacement
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1. Standarization of NaOH with Potassium Hydrogenphthalate (KHC8H404) The hygroscopic nature of NaOH requires its solution to be standardized with a stable primary standard such as potassium hydrogen phthalate. The exact molar concentration of NaOH solution can then be accurately determined. 1.1) Prepare the stock NaOH solution by dissolving about 4 g of NaOH pellet in 100 mL of deionized water. Note that NaOH is a hazardous chemical which is corrosive to skin and irritant to eye‚ be cautious and
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