A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired analyte is passed
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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Eric McKenzie Geophysical Science Honors February 14th‚ 2014 ACID RAIN 1. Acid rain is rainfall created by pollution in the atmosphere. When the rainfall occurs‚ it almost always causes harm to the environment. 2. Acid rain is caused by the release of certain compounds into the air‚ including sulfur dioxide and nitrogen oxides. These compounds mix and react with chemicals such as oxygen and water and form acid rain. 3. Acid rain can harm the environment by acidifying lakes and streams‚ which greatly
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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Melting pointe determination #01 Introduction: Objective To learn how to obtain an accurate melting point using a MELTING POINT APPARATUS‚ then use them to draw eutectic curve and determine the eutectic point. Melting point of a solid is the temperature at which the solid and its liquid form are in equilibrium‚ i.e.‚ molecules move back and forth between the two states at the same rate‚ so both phases remain present. If the temperature of a solid is measured carefully as the solid is heated
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Farrell cengage.com/chemistry/campbell Chapter Nine Nucleic Acids: How Structure Conveys Information Paul D. Adams • University of Arkansas Nucleic Acids • Nucleic acid: a biopolymer containing three types of monomer units • a base derived from purine or pyrimidine (nucleobases) • a monosaccharide‚ either D-ribose or 2-deoxy-D-ribose • phosphoric acid • RNA (Ribonucleic Acid) • DNA (Deoxyribonucleic Acid) Nucleic Acids • Levels of structure • 1°structure: the order of bases on
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Chapter 4: Nucleic Acids and the RNA World 1. 4.1 – What is a Nucleic Acid? * Nucleic acids are made up of monomers called nucleotides * Three components of a nucleotide: 1. Phosphate group—attached to the 5’ carbon 2. Sugar – carbonyl group and several hydroxyl groups 3. Nitrogenous base * The prime (‘) symbols indicate the carbon being is part of the sugar—not attached to the nitrogenous base. * Four different nucleotides‚ each of which contains a different nitrogenous
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ACID/BASE You might need to know the following K values: CH3COOH Ka = 1.8 x 10–5 Benzoic Acid Ka = 6.5 x 10–5 HNO2 Ka = 4.5 x 10–4 NH3 Kb = 1.8 x 10–5 HF Ka = 7.2 x 10–4 H2S Ka = 5.7 x 10–8 HSO4– Ka = 1.2 x 10–2 HS– Ka = 1.2 x 10–13 HCOOH Ka = 1.8 x 10–4 HOCl Ka = 3.0 x 10–8 SIMPLE ACIDS AND BASES 1. According to the Brønsted–Lowry definition‚ which species can function both as an acid and as a base? (A) Cl– (B) SO42– (C) NH4+ (D) HCO3– (E) H3O+ 2. Which of the following
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Determination of Chemical Formulae: The Reaction of Zinc and Iodine By Sarah Abstract: The main objective of this experiment was to use to the reaction between zinc and iodine to examine the validity of the Law of Conservation of Mass and the Law of Constant Composition. The Law of Constant Composition was tested by determining the mass of each of the reactants‚ zinc and iodine‚ and comparing their total to the mass of the zinc iodide product plus the excess zinc. The total mass of the reactants
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Acid Rain Acid rain occurs when Sulphur Dioxide (SO2) is released into the atmosphere by electricity generation‚ motor vehicles‚ factories and by wildfires and volcanoes. Once released‚ the sulphur dioxide reacts with water to produce sulphuric acid. This has a pH of approximately 2-3 pH. This has a dramatic effect on water dwelling wildlife. Most water dwelling creatures can’t withstand water more acidic than pH 5. This causes the mass loss of life of animals such as trout‚ perch‚ frogs and mayflies
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