Microbiology Lab Report II Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior
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Title:Extraction:Extraction with acid and alkaline Objective: 1. To recover benzoic acid and p-dichlorobenzene from its mixture using acid-alkaline extraction. 2. To determine the percentage recovery and melting point of benzoic acid and p-diclorobenzene. Apparatus:Separatory funnel(250mL)‚Buchner funnel‚beaker. Materials:Benzoic acid‚p-dichlorobenzene‚ether‚10% NaOH‚conc.HCl‚distilled water‚ anhydrous CaCl2. Introduction: Organic compounds in an aqueous mixture can be separated
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Acid rain‚ or more accurately acid precipitation‚ is the term used for describing rainfall with a pH level lower than 5.6. This type of pollution is a matter of great debate currently due to the potential of its causing environmental damages all across the world. For the last decade or so acid rain has caused destruction to hundreds of lakes and streams in many parts of the world‚ including the US‚ Canada‚ and Europe. Acid rain forms due to the oxides of sulfite and nitrogen combining with the moisture
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ACID BASE IMBALENCE INTRODUCTION The body normally maintains a steady balance between acids‚ produced during metabolism and bases that neutralize and promote the excretion of the acids.Many health problems may lead to acid base imbalance.Patients with Diabetis mellitus ‚COPD‚and kidney disease frequently frequently develop acid base imbalences. Vomiting and diarrhea may also cause acid base imbalance.The kidneys are an essential buffer system for
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What are trans fatty acids‚ and where do they come from? We’re used to hearing about saturated and unsaturated fatty acids. Saturated fatty acids - which come from animal fats (meat‚ lard‚ dairy products) as well as tropical oils such as coconut and palm oils - raise the levels of LDL cholesterol. Unsaturated fats - which come from vegetable oils - in general‚ do not increase cholesterol levels‚ and may reduce them. Because saturated fatty acids were found to be bad for you a couple decades ago
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quantity of solid dissolved in solution. It may also be used to calculate the molar mass of an unknown acid or base. Purpose: I. Prepare a standard solution of sodium hydroxide II. Standardize a sodium hydroxide solution by using potassium hydrogen phthalate (KHP) III. When given whether the acid is monoprotic‚ diprotic‚ or triprotic ‚ determine the molar mass of the unknown organic acid. IV. Calculate the Molarity of the Soft drink. Materials: * 50 mL Buret * Beaker
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solution of an acidic and organic compound and purify its respective acidic product through the techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through the Hirsch funnel into separate beakers of an aqueous
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The Red Line The story “The Red Line” by Charles Higson revolves around a woman and two men of whom one is stabbed and another becomes a murderer. All of the characters share the feeling of displacement. They all feel different from other people. The first character we meet‚ the Bob Seeger imitator‚ feel that he is different from others in his appearance and better than others when it comes to performance. “when he looked out of the window he could see people. Ugly people.”. He thinks that people
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Titration Lab How Much Citric Acid is in Your Soda? PURPOSE To determine the molarity (concentration) of citric acid (H3C6H5O7) in various different citrus flavored sodas. BACKGROUND PRINCIPLES Popular sodas all strive for a somewhat sour (’tart’) flavor and manufacturers utilize acids to impart this taste. While there are a vast variety of different brands of sodas on the market‚ they can be broken into two main categories: dark or clear. This differentiation is not only a simple color
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Crystallization of Benzoic Acid Purpose: The purpose of this experiment is to purify benzoic acid by crystallization. Procedure: 1. 0.5g impure benzoic acid was placed in a 50mL Erlenmeyer flask. 15mL of water was added and the mixture was heated to a boil on a hot plate. 0.5mL of water was added to the flask‚ while swirling the flask. The mixture was boiled until the benzoic acid completely dissolved. The total volume of water used was recorded. The black solid that remain in the dissolved
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