Experiment 1 Lab Report Title: Determination of caffeine content in tea bag sample by UV spectroscopic method Higher diploma in Pharmaceutical Science-Year 1 AS114202-1TM Fundamental Analytical Chemistry ASD3008 Lab Group 6: Ng Chung Hin 130439161 Wong Tin Yau Tony 130252529 Ng Wai Kin 130031247 Tam Chun Kit 130191034 Mau Kwan Yiu 130395762 Date of Experiment: 7/3/2014 Content Introduction Objectives Methodology Results Discussion Conclusions References Introduction
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Trace Evidence Trace evidence is an important part of a team in solving crimes in forensic investigations. According to Edmond Locard‚ there is specific that no matter wherever people interact with their environment or is physically involved in a crime often leaves something at or something away from the scene. Those in this category of evidence include many diverse types of macroscopic or microscopic materials that certain examples are easily visible to our “naked eye”. The subject is broad and
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and sampling techniques Spectrophotometer Spectrophotometry in chemistry is a quantitative measure of the reflection or transmission of material properties as a function of wavelength. It is more specialized than the term electromagnetic spectroscopy which is general in that spectrophotometry deals with near-ultraviolet‚ visible light‚ and near-infrared‚ but doesn’t cover the techniques of time-resolved spectroscopic . Spectrophotometer is used in Spectrophotometry. A spectrophotometer is
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Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the use of electromagnetic radiation by a sample in order to identify components of
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CH 202 Breakdown UV/Vis: Molecules containing π-electrons or non-bonding electrons (n-electrons) can absorb the energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals. The more easily excited the electrons (i.e. lower energy gap between the HOMO and the LUMO)‚ the longer the wavelength of light it can absorb. Conjugation raises the energy of the bonding orbitals and lowers the energy of the antibonding molecular orbital. This
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atoms and molecules are determined. This technique is spectroscopy where it absorbs‚ emits‚ or scatters electromagnetic radiation. Electromagnetic radiation has particle-like properties having photons and quantum and wave-like properties with frequency and wavelength. To make molecules visible through light‚ the wavelength of the molecule should be longer than the wavelength of the light. One spectroscopic
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Background NMR spectroscopy is a well established method for structure determination of various organic molecules ranging from small molecules all the way up to large bio-molecules such as proteins‚ DNA and polysaccharides. The chemical environment surrounding each atom as well their proximity to other atoms in a molecule can be studied with various experiments‚ enabling high-resolution 3D-structure determinations as well as verification of the chemical structure. NMR spectroscopy is well suited
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Module 2 Mass Spectroscopy [pic] [pic] Diagram of a mass spectrometer Stage 1: Ionisation The sample is injected as a vapour and the atoms are bombarded by fast moving electrons. This causes the molecule to fragment into two or more pieces. When a molecule or part thereof fragments‚ one portion would be positively charged while the second portion will be uncharged. All uncharged fragments are ultimately lost in the machine as mass spectrometers only work with positive ions. Stage 2: Acceleration
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Callam 1/8 CHEM – 254 – WI -2009 Problem Set #1 – IR Spectroscopy - KEY 1. Which of the indicated bonds absorbs at the higher frequency in an IR spectrum? a. O or OH C=O > C-O b. N or NH C=N > C-N c. H or H C-H sp2 > C-H sp3 Callam 2/8 CHEM – 254 – WI -2009 2. What major IR absorptions are present above 1500 cm-1 for each compound? a. b. O sp3 C-H stretch 2950 C=O - ketone 1715 cm-1 cm-1 c. O sp3 C-H stretch 2950 cm-1 sp3 C-H stretch 2950 cm-1
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Lab Report 4 Biology 103 Prof. Dr. Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of
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