De Leon‚ Jo-Anna Maria N. 3ChEA Group No. 2 Experiment No. 5 Spectrophotometric Determination of pKa I. Introduction The equilibrium constant of the dissociation of an indicator in water will be determined experimentally using spectroscopic method.This experiment aims to demonstrate the principle of equilibrium and the interaction of matter with light. II. Methodology Figure . UV-VIS Spectrophotometer 10 solutions covering the entire pH range from 1 to 13 were prepared
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Beer’s Law Lab Hanna Beeter March 15‚ 2013 Honors Chemistry Introduction: In Beer’s law lab the purpose was to find the unknown concentration. The lab included the use of a volumetric flask‚ pipette‚ and spectrophotometer. The spectrophotometer played the biggest part in finding the concentration of the unknown. A spectrophotometer is an apparatus for measuring the intensity of light in a part of the spectrum‚ and is transmitted or emitted on a particular substance. After the number retrieved
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2.5. Characterization Methods 1H–NMR and 13C–NMR spectra of HPPP‚ BOMP and HVPA were taken in CDCl3 on Bruker DPX–300 MHz spectrometer using tetramethylsilane (TMS) as an internal standard. FT–IR spectra of the synthesized compounds‚ HPPP‚ BOMP‚ HVPA and photocrosslinked polymers were recorded on Perkin-Elmer system 2000 (4000–400 cm–1) spectrometer‚ using KBr pellets. Medium pressure mercury vapour lamp to the power output of 125W/cm2 was used for the photocrosslinking study. The viscosity of the
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1.1 DETECTION OF Hg2+ Refer(1) Why is detection of Hg2+ important?? Mercury contamination poses severe health risks. It can enter into environment through natural as well as human activities. Once it enters marine environment‚ bacteria convert inorganic mercury to methyl mercury which enters food chain and is accumulated in large edible fish. Its imbalance can lead to neurological disorders and can cause Minamata disease. Procedure to detect Hg2+ 1. MS1 (Mercury Sensor 1)‚ a water soluble‚ fluorescein
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the science lab. In science‚ we would participate in a lab. One of my favorite labs was the owl pellet dissection. Owl pellets are owl throw up. In owl pellets you can find the bones of animals the owl consumed. In this lab we were to take out the bones we found in the pellet and construct the animal we found inside. Most people‚ like me‚ had a sort of rodent‚ but some people found birds in their pellet. Another one of my favorite things we did in science was the Sharpie tie dye lab in fifth grade
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Experiment # 9: Optical Method of Analysis Use of Beer’s Law on a KMn04 Gregorio‚ Justin Edrik A. March 2013 Department of Chemical Engineering‚ Faculty of Engineering University of Santo Tomas España‚ Manila Abstract The purpose of this analytical laboratory experiment is to determine the unknown concentration of potassium permanganate (KMnO4) solution by finding its absorbance through the use of spectrophotometer. The preparation of four known concentration of KMnO4 was done namely‚ 2
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Australian physicist Alan walah Atomic spectrometric methods are rapid ‚convenient and have high selectivity The method involves a process called as atomization in which the sample is converted to a gaseous phase atoms or ions Atomic absorption spectroscopy is based on the same principle as the flame test used in qualitative analysis. When an alkali metal salt or a calcium‚ strontium or barium salt is heated strongly in the Bunsen flame‚ a characteristic flame colour is observed: Na -- yellow Li--
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The pigments called chlorophylls in the plants are what trap the light energy. Carotenoids also help aid the chlorophylls by also absorbing light. The two chlorophylls found in green plants are chlorophyll a and chlorophyll b. The purpose of this lab is to extract the pigments from spinach leaves and separate them by using chromatography to determine each of the pigments absorption spectra. Methods and Materials For this experiment I used a 10mL pipette‚ a 100mL graduated cylinder‚ a rubber
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The Visible Spectra Of Orange Soda Pop Objective : To measure an absorbance value of the different colour solutions by using Spectrophotometry Introduction Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color‚ or more specifically‚ the wavelength of light (nm). The instrument also can be use on biological sample such as chlorophyll pigments and
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Spectrophotometry was used in the lab to determine whether non-magnetized zeolite‚ magnetized zeolite‚ or charcoal was the more effective sequestration agent for Procion Red Dye. A calibration curve was created with the known concentrations and the absorbances of the Procion Red Dye dilutions at λmax. The slope of the calibration curve was used to determine the concentration of the analytes. As a result‚ charcoal was shown to be the more effective sequestration agent. Introduction PAHs‚ polycyclic
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